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Plant Gene Silencing

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Kirankumar S. Mysore

    1. Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Muthappa Senthil-Kumar

    1. National Institute of Plant Genome Research, New Delhi, India

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2408)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Recent Advances in Plant Gene Silencing Methods

    • Prachi Pandey, Kirankumar S. Mysore, Muthappa Senthil-Kumar
    Pages 1-22

  3. Strategies for Efficient RNAi-Based Gene Silencing of Viral Genes for Disease Resistance in Plants

    • Krish K. Kumar, Shanmugam Varanavasiappan, Loganathan Arul, Easwaran Kokiladevi, Duraialagaraja Sudhakar
    Pages 23-35

  4. Genome Editing and Designer Crops for the Future

    • Sumi Rana, Pooja Rani Aggarwal, Varsa Shukla, Urmi Giri, Shubham Verma, Mehanathan Muthamilarasan
    Pages 37-69

  5. In Silico Methods for the Identification of Viral-Derived Small Interfering RNAs (vsiRNAs) and Their Application in Plant Genomics

    • Aditya Narayan, Shafaque Zahra, Ajeet Singh, Shailesh Kumar
    Pages 71-84

  6. Barley Stripe Mosaic Virus (BSMV)-Based Virus-Induced Gene Silencing to Functionally Characterize Genes in Wheat and Barley

    • Harvinder Bennypaul, Upinder S. Gill
    Pages 85-93

  7. Virus-Induced Gene Silencing in Wheat and Related Monocot Species

    • Vinay Panwar, Kostya Kanyuka
    Pages 95-107

  8. Virus-Induced Gene Silencing in Sorghum Using Brome Mosaic Virus

    • Dharmendra K. Singh, Kirankumar S. Mysore
    Pages 109-115

  9. RTBV-Based VIGS Vector for Functional Genomics in Rice: Methodology, Advances, Challenges, and Future Implications

    • Gaurav Kumar, Kamlesh Kumari, Indranil Dasgupta
    Pages 117-131

  10. Optimization of Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing (VIGS) in Tomato

    • Ashish Kumar Singh, Dibyendu Ghosh, Supriya Chakraborty
    Pages 133-145

  11. Virus-Induced Gene Silencing for Functional Genomics of Specialized Metabolism in Medicinal Plants

    • Dikki Pedenla Bomzan, Krishna Kumar, Sarma Rajeev Kumar, Seema Meena, Dinesh A. Nagegowda
    Pages 147-163

  12. VIGS-Based Gene Silencing for Assessing Mineral Nutrient Acquisition

    • â–ª Akash, Rajat Srivastava, Rahul Kumar
    Pages 165-179

  13. High-Throughput Analysis of Gene Function under Multiple Abiotic Stresses Using Leaf Disks from Silenced Plants

    • Ramegowda Yamunarani, Venkategowda Ramegowda, Muthappa Senthil-Kumar, Kirankumar S. Mysore
    Pages 181-189

  14. A Method for Developing RNAi-Derived Resistance in Cowpea Against Geminiviruses

    • Sanjeev Kumar, Sunil Kumar Mukherjee, Lingaraj Sahoo
    Pages 191-210

  15. In Vitro Method for Synthesis of Large-Scale dsRNA Molecule as a Novel Plant Protection Strategy

    • Siddappa Sundaresha, Aarti Bairwa, Maharishi Tomar, Ravinder Kumar, E. P. Venkatasalam, Vinay Sagar et al.
    Pages 211-226

  16. Fine-Tuning Plant Gene Expression with Synthetic Trans-Acting Small Interfering RNAs

    • Lucio López-Dolz, Maria Spada, José-Antonio Daròs, Alberto Carbonell
    Pages 227-242

  17. Trans-Kingdom RNA Silencing in Plant-Fungal Disease Control

    • Tao Zhang, Fei Wang, Hui-Shan Guo, Yun Jin
    Pages 243-252

  18. An Integrated Bioinformatics and Functional Approach for miRNA Validation

    • Sombir Rao, Sonia Balyan, Chandni Bansal, Saloni Mathur
    Pages 253-281

  19. Development of a Ligation-Independent Cloning-Based Dual Vector System for RNA Interference in Plants

    • Jinping Zhao, Carlos Garcia Rios, Jingjing Xu, Ijaz Ahmad, Junqi Song
    Pages 283-292

  20. Multigene Transformation Through Cre-lox Mediated Site-Specific Integration in Rice

    • Bhuvan Pathak, Soumen Nandy, Vibha Srivastava
    Pages 293-302
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Keywords

About this book

This second edition provides a comprehensive review of various gene silencing methodologies and applications. Chapters detail a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS), understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, artificial miRNAs for gene silencing, and high throughput RNAi. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Plant Gene Silencing: Methods and Protocols, Second Edition aims to further the understanding of functional relevance of target genes using gene silencing methods and technologies in commercial plant varieties. 



Editors and Affiliations

  • Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, USA

    Kirankumar S. Mysore

  • National Institute of Plant Genome Research, New Delhi, India

    Muthappa Senthil-Kumar

Bibliographic Information

  • Book Title: Plant Gene Silencing

  • Book Subtitle: Methods and Protocols

  • Editors: Kirankumar S. Mysore, Muthappa Senthil-Kumar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1875-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1874-5Published: 25 March 2022

  • Softcover ISBN: 978-1-0716-1877-6Published: 26 March 2023

  • eBook ISBN: 978-1-0716-1875-2Published: 24 March 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 328

  • Number of Illustrations: 7 b/w illustrations, 49 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics

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