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NF-κB Transcription Factors

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Guido Franzoso

    1. Centre for Molecular Immunology and Inflammation, Department of Immunology and Inflammation, Imperial College London, London, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Francesca Zazzeroni

    1. Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, L’Aquila, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2366)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xvi
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  2. General Methods for Analyzing NF-κB Activation

    1. Front Matter

      Pages 1-1
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    2. Measuring NF-κB Phosphorylation and Acetylation

      • Nikita Tushar Modi, Lin-Feng Chen
      Pages 3-17

    3. Biochemical Methods to Analyze the Subcellular Localization of NF-κB Proteins Using Cell Fractionation

      • Davide Vecchiotti, Daniela Verzella, Daria Capece, Mauro Di Vito Nolfi, Barbara Di Francesco, Jessica Cornice et al.
      Pages 19-25

    4. Immunohistochemical Analysis of Expression, Phosphorylation, and Nuclear Translocation of NF-κB Proteins in Human Tissues

      • Davide Vecchiotti, Daniela Verzella, Daria Capece, Jessica Cornice, Mauro Di Vito Nolfi, Barbara Di Francesco et al.
      Pages 27-42

    5. High-Throughput Analysis of the Cell and DNA Site-Specific Binding of Native NF-κB Dimers Using Nuclear Extract Protein-Binding Microarrays (NextPBMs)

      • Heather Hook, Rose W. Zhao, David Bray, Jessica L. Keenan, Trevor Siggers
      Pages 43-66

    6. Molecular and Biochemical Approaches to Study the Evolution of NF-κB Signaling in Basal Metazoans

      • Pablo J. Aguirre Carrión, Leah M. Williams, Thomas D. Gilmore
      Pages 67-91

  3. Methods for Studying the Activation of NF-κB Downstream of Distinct Signaling Pathways

    1. Front Matter

      Pages 93-93
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    2. Methods for Modulating the Pathway of NF-κB Using Short Hairpin RNA (ShRNA)

      • Marta Moretti, Barbara Di Francesco, Mauro Di Vito Nolfi, Annapaola Angrisani, Enrico De Smaele
      Pages 95-107

    3. Immunoblot Analysis of the Regulation of TNF Receptor Family-Induced NF-κB Signaling by c-IAP Proteins

      • Eugene Varfolomeev, Tatiana Goncharov, Domagoj Vucic
      Pages 109-123

    4. Methods to Study CARD11-BCL10-MALT1 Dependent Canonical NF-κB Activation in Jurkat T Cells

      • Andreas Gewies, Carina Graß, Daniel Krappmann
      Pages 125-143

    5. Analysis of Calcium Control of Canonical NF-κB Signaling in B Lymphocytes

      • Corbett T. Berry, Michael J. May, Bruce D. Freedman
      Pages 145-164

    6. A Kinase Assay for Measuring the Activity of the NIK-IKK1 Complex Induced via the Noncanonical NF-κB Pathway

      • Tapas Mukherjee, Yashika Ratra, Balaji Banoth, Alvina Deka, Smarajit Polley, Soumen Basak
      Pages 165-181

    7. Analyze the SUMOylation of IKK γ/NEMO During Genotoxic Stress

      • Zhao-Hui Wu, Shigeki Miyamoto
      Pages 183-190

  4. Methods for Analyzing NF-κB Activation in Physiology and Disease

    1. Front Matter

      Pages 191-191
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    2. Analysis of the Contribution of NF-κB in the Regulation of Chemotherapy-Induced Cell Senescence by Establishing a Tetracycline-Regulated Cell System

      • Francesco Pacifico, Elvira Crescenzi, Antonio Leonardi
      Pages 193-212

    3. Methods to Detect NF-κB Activity in Tumor-Associated Macrophage (TAM) Populations

      • Fan Sun, Zhaoxia Qu, Gutian Xiao
      Pages 213-241

    4. Methods to Study the Effect of IKK Inhibition on TNF-Inducing Apoptosis and Necroptosis in Cultured Cells

      • Alessandra Pescatore, Carmela Casale, Francesca Fusco, Matilde Valeria Ursini
      Pages 243-254

    5. Use of ChIP-qPCR to Study the Crosstalk Between HIF and NF-κB Signaling in Hypoxia and Normoxia

      • Dilem Shakir, Michael Batie, Sonia Rocha
      Pages 255-265

    6. Methods to Analyze the Roles of TAK1 , TRAF6, and NEMO in the Regulation of NF-κB Signaling by RANK Stimulation During Osteoclastogenesis

      • Gaurav Swarnkar, Manoj Arra, Suresh Adapala, Yousef Abu-Amer
      Pages 267-282
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Keywords

About this book

This detailed book serves as a systematic examination of the analytical methods to study the transcription factor NF-κB in physiology and disease. It provides an up-to-date guidebook to navigate both conventional and highly specialized methods to detect and analyze the different signaling pathways of NF-κB activation and contextualize them within organismal physiology and disease pathogenesis, using genetic and biochemical techniques and some of the most advanced computational and systems biology methods. Additionally, the volume includes several examples of approaches utilized by leading experts in the NF-κB field to analyze and modulate NF-κB signaling in specific physiological and disease contexts, along with some of the most promising approaches to pharmacologically target the NF-κB pathway in human disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, NF-κB Transcription Factors: Methods and Protocols is an up-to-date guide intended for both basic and translational scientists who are working in the dynamic NF-κB field.

Editors and Affiliations

  • Centre for Molecular Immunology and Inflammation, Department of Immunology and Inflammation, Imperial College London, London, UK

    Guido Franzoso

  • Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, L’Aquila, Italy

    Francesca Zazzeroni

Bibliographic Information

  • Book Title: NF-κB Transcription Factors

  • Book Subtitle: Methods and Protocols

  • Editors: Guido Franzoso, Francesca Zazzeroni

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1669-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1668-0Published: 09 July 2021

  • Softcover ISBN: 978-1-0716-1671-0Published: 09 July 2022

  • eBook ISBN: 978-1-0716-1669-7Published: 08 July 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 359

  • Number of Illustrations: 22 b/w illustrations, 38 illustrations in colour

  • Topics: Immunology, Genetics and Genomics

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