Editors:
- Includes cutting-edge methods and protocol
- Provides step-by-step detail essential for reproducible result
Part of the book series: Methods in Molecular Biology (MIMB, volume 2357)
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Table of contents (19 protocols)
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Front Matter
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Introduction
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Front Matter
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Quantification and Enrichment of Persister Cells
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Front Matter
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Single-Cell Analysis of Persister Cells
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Front Matter
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Omics Approaches in Persistence Research
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Front Matter
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Persister Cell Resuscitation and Eradication
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Front Matter
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About this book
This volume presents a collection of methods that have contributed to the current understanding of bacterial persisters. Chapters in the book detail general guidelines for measuring persister levels in bacterial cultures, strategies to enrich and resuscitate persister subpopulations, single-cell approaches for visualizing and characterizing persisters, omics techniques and cellular and animal models for studying persistence. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols, Second Edition aims to be a useful practical guide to researchers to help further their study in this field.
Editors and Affiliations
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VIB-KU Leuven Center for Microbiology, VIB, Leuven, Belgium
Natalie Verstraeten, Jan Michiels
Bibliographic Information
Book Title: Bacterial Persistence
Book Subtitle: Methods and Protocols
Editors: Natalie Verstraeten, Jan Michiels
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1621-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2021
Hardcover ISBN: 978-1-0716-1620-8Published: 30 September 2021
Softcover ISBN: 978-1-0716-1623-9Published: 01 October 2022
eBook ISBN: 978-1-0716-1621-5Published: 29 September 2021
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XI, 292
Number of Illustrations: 9 b/w illustrations, 42 illustrations in colour
Topics: Immunology, Bacteriology