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Peptide Conjugation

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Waleed M. Hussein

    1. School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Rachel J. Stephenson

    1. School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Australia

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    You can also search for this editor in PubMed   Google Scholar

  3. Istvan Toth

    1. School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Australia

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2355)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Peptide-Polymer Conjugation Via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition

    • Waleed M. Hussein, Istvan Toth, Mariusz Skwarczynski
    Pages 1-7

  3. Conjugation of Peptides to Gold Nanoparticles

    • Pornsuda Maraming, James Chen Yong Kah
    Pages 9-16

  4. Peptide-Protein Conjugation and Characterization to Develop Vaccines for Group A Streptococcus

    • Sharareh Eskandari, Michael F. Good, Manisha Pandey
    Pages 17-33

  5. Oxime/Hydrazone Conjugation at Histidine: Late-Stage Functionalization Approach of Unprotected Peptides

    • Anaïs F. M. Noisier, Ranganath Gopalakrishnan
    Pages 35-48

  6. Modification of Nanoparticles with Transferrin for Targeting Brain Tissues

    • Sara Aly Attia, Xiang Li, Nina Filipczak, Daniel F. Costa, Vladimir P. Torchilin
    Pages 49-56

  7. Peptide-Pegylated Lipid Conjugation Via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition

    • Waleed M. Hussein, Istvan Toth
    Pages 57-64

  8. Vitamin B12 – Peptide Nucleic Acid Conjugates

    • Aleksandra J. Wierzba, Monika Wojciechowska, Joanna Trylska, Dorota Gryko
    Pages 65-82

  9. Enzymatic Ligation of Disulfide-Rich Animal Venom Peptides: Using Sortase A to Form Double-Knotted Peptides

    • Poanna Tran, Christina I. Schroeder
    Pages 83-92

  10. Solid-State, Thermal Synthesis of Peptide/Protein–Boron Cluster Conjugates

    • Krzysztof Fink, Tomasz M. Goszczyński
    Pages 93-104

  11. Bioconjugation of Peptides to Hybrid Gold Nanoparticles

    • Rosalba Moretta, Monica Terracciano, Ilaria Rea, Luca De Stefano
    Pages 105-115

  12. Design and Synthesis of a Peptide-Based Glioma-Targeted Drug Delivery Vector gHope2

    • Kaido Kurrikoff, Nikita Oskolkov, Elo Eriste, Ülo Langel
    Pages 117-129

  13. Electrochemically Enabled C-Terminal Peptide Modifications

    • Yutong Lin, Lara R. Malins
    Pages 131-139

  14. Double Conjugation Using Mercapto-Acryloyl and Alkyne-Azide Reactions for the Synthesis of Branched Multiantigenic Vaccine Candidates

    • Waleed M. Hussein, Mariusz Skwarczynski, Istvan Toth
    Pages 141-150

  15. Chemical Protein Synthesis by Chemoselective #x03B1;-Ketoacid–Hydroxylamine (KAHA) Ligations with 5-Oxaproline

    • Jakob Farnung, Haewon Song, Jeffrey W. Bode
    Pages 151-162

  16. Site-Specific Modification of Single-Chain Affinity Ligands for Fluorescence Labeling, Radiolabeling, and Bioconjugation

    • Boya Zhang, Sachith M. Vidanapathirana, Colin F. Greineder
    Pages 163-173

  17. Preparation and Characterization of Quantum Dot-Peptide Conjugates Based on Polyhistidine Tags

    • Katherine D. Krause, Hsin-Yun Tsai, Kelly Rees, Hyungki Kim, W. Russ Algar
    Pages 175-218

  18. The Construction of a Genetically Encoded, Phage-Displayed Cyclic-Peptide Library

    • Peng-Hsun Chase Chen, Wenshe Ray Liu
    Pages 219-230

  19. The Chemical Synthesis of Site-Specifically Modified Proteins Via Diselenide-Selenoester Ligation

    • Rhys C. Griffiths, Nicholas J. Mitchell
    Pages 231-251

  20. Synthesis of Lipopeptides by CLipPA Chemistry

    • Victor Yim, Yann O. Hermant, Paul W. R. Harris, Margaret A. Brimble
    Pages 253-263
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Keywords

About this book

This volume explores diverse protocols for peptide conjugation, and provides thoroughly tested and scientifically valid techniques that allow researchers and scientists to prepare, purify, characterize, and use peptide conjugation methods for chemical, biochemical, and biological studies. Some of the topics discussed in this book are gold nanoparticles, proteins, pegylated lipids, and vitamins. Chapters also cover enzymatic ligation using sortase A, construction of a phage-displayed cyclic-peptide library, quantum dot-peptide conjugates, and preparation of lipopeptides by CLipPA chemistry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Cutting-edge and comprehensive, Peptide Conjugation: Methodsand Protocols is a valuable resource for experienced researches and undergraduate students alike who are interested in learning more about this exciting and developing field. 


Editors and Affiliations

  • School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Australia

    Waleed M. Hussein, Rachel J. Stephenson, Istvan Toth

Bibliographic Information

  • Book Title: Peptide Conjugation

  • Book Subtitle: Methods and Protocols

  • Editors: Waleed M. Hussein, Rachel J. Stephenson, Istvan Toth

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1617-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1616-1Published: 14 August 2021

  • Softcover ISBN: 978-1-0716-1619-2Published: 15 August 2022

  • eBook ISBN: 978-1-0716-1617-8Published: 12 August 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 324

  • Number of Illustrations: 20 b/w illustrations, 59 illustrations in colour

  • Topics: Biochemistry, general, Biological Techniques

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