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Magnaporthe oryzae

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Stefan Jacob

    1. Institute of Biotechnology and Drug Research gGmbH (IBWF), Mainz, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2356)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. The Impact of Blast Disease: Past, Present, and Future

    • Barbara Valent
    Pages 1-18

  3. The Biology of Invasive Growth by the Rice Blast Fungus Magnaporthe oryzae

    • Neftaly Cruz-Mireles, Iris Eisermann, Marisela Garduño-Rosales, Camilla Molinari, Lauren S. Ryder, Bozeng Tang et al.
    Pages 19-40

  4. Analysis of the Secondary Metabolism in Magnaporthe oryzae

    • Elizabeth Skellam
    Pages 41-56

  5. The Determination of Carbohydrates by High-Performance Anion-Exchange Chromatography Coupled with Pulsed Amperometric Detection (HPAEC-PAD)

    • Christiane Grünewald, Stefan Bohnert, Stefan Jacob
    Pages 57-68

  6. Characterizing the Secretion Systems of Magnaporthe oryzae

    • Ely Oliveira-Garcia, Barbara Valent
    Pages 69-77

  7. Specimen Preparation and Observations of Magnaporthe oryzae Appressorial Cells Under Electron Microscopy

    • Raquel O. Rocha, Richard A. Wilson
    Pages 79-85

  8. 4D Widefield Fluorescence Imaging of Appressorium Morphogenesis by Magnaporthe oryzae

    • Audra Rogers, Nawaraj Dulal, Martin Egan
    Pages 87-96

  9. Tandem Affinity Purification (TAP) of Low-Abundance Protein Complexes in Filamentous Fungi Demonstrated Using Magnaporthe oryzae

    • Gang Li, Richard A. Wilson
    Pages 97-108

  10. Quantitative Proteome and Phosphoproteome Profiling in Magnaporthe oryzae

    • Thomas Michna, Stefan Tenzer
    Pages 109-119

  11. A Target-Based In Vivo Test System to Identify Novel Fungicides with Mode of Action in the HOG Pathway

    • Stefan Bohnert, Hendrik Neumann, Stefan Jacob
    Pages 121-127

  12. Making Visualization Work for You: Deriving Valuable Insights from Omics Data

    • Alexander Yemelin
    Pages 129-148

  13. Genome Editing Using CRISPR/Cas9 System in the Rice Blast Fungus

    • Takayuki Arazoe
    Pages 149-160

  14. Diurnal, Circadian , and Photomorphogenic Analyses in Magnaporthe oryzae

    • Ciarán Griffin, George R. Littlejohn
    Pages 161-172

  15. Methods to Study Autophagocytosis in Magnaporthe oryzae

    • Lin Li, Xue-Ming Zhu, Xiao-Hong Liu, Fu-Cheng Lin
    Pages 173-185

  16. Molecular Detection of Magnaporthe oryzae from Rice Seeds

    • Sonia Chadha
    Pages 187-197

  17. Isolation and Functional Analysis of Effector Proteins of Magnaporthe oryzae

    • Meng-Di Dai, Yu Li, Li-Xiao Sun, Fu-Cheng Lin, Xiao-Hong Liu
    Pages 199-209

  18. Analysis of Genetic Variations and Genomic Instabilities in Magnaporthe oryzae

    • Sonia Chadha
    Pages 211-224

  19. Controllable Bypass Suppression in Magnaporthe oryzae

    • Stefan Jacob, Katharina Bersching
    Pages 225-231

  20. Back Matter

    Pages 233-234
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Keywords

About this book

This volume highlights molecular methods to study the phytopathogenic rice blast fungus Magnaporthe oryzae. Chapters in this book cover the history, development, and evolution of the pathogen; molecular methods to increase the knowledge of the biology, genetic, and metabolic diversity of the pathogen; and the pathogen’s adaptability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Magnaporthe oryzae: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this developing field. 


Editors and Affiliations

  • Institute of Biotechnology and Drug Research gGmbH (IBWF), Mainz, Germany

    Stefan Jacob

Bibliographic Information

  • Book Title: Magnaporthe oryzae

  • Book Subtitle: Methods and Protocols

  • Editors: Stefan Jacob

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1613-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1612-3Published: 09 July 2021

  • Softcover ISBN: 978-1-0716-1615-4Published: 09 July 2022

  • eBook ISBN: 978-1-0716-1613-0Published: 08 July 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 234

  • Number of Illustrations: 15 b/w illustrations, 39 illustrations in colour

  • Topics: Plant Pathology, Mycology

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