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Solanum tuberosum

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. David Dobnik

    1. Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia

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  2. Kristina Gruden

    1. Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia

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  3. Živa Ramšak

    1. Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia

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  4. Anna Coll

    1. Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia

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  • Includes cutting-edge methods and protocol
  • Provides step-by-step detail essential for reproducible result
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2354)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
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  2. History, Breeding and Long-term Storage

    1. Front Matter

      Pages 1-1
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    2. Importance of Potato as a Crop and Practical Approaches to Potato Breeding

      • Peter Dolničar
      Pages 3-20

    3. Cryopreservation of Potato Shoot Tips for Long-Term Storage

      • Rainer Vollmer, Janeth Espirilla, Rosalva Villagaray, José Cárdenas, Mario Castro, Juan Carlos Sánchez et al.
      Pages 21-54Open Access

  3. High-throughput Omics Techniques

    1. Front Matter

      Pages 55-55
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    2. RNA Sequencing Analyses for Deciphering Potato Molecular Responses

      • Živa Ramšak, Marko Petek, Špela Baebler
      Pages 57-94

    3. Yeast Two-Hybrid Screening for Identification of Protein-Protein Interactions in Solanum tuberosum

      • Hazel McLellan, Miles R. Armstrong, Paul R. J. Birch
      Pages 95-110

    4. Potato as a Model for Field Trials with Modified Gene Functions in Research and Translational Experiments

      • Nam Phuong Kieu, Marit Lenman, Erik Andreasson
      Pages 111-122

    5. DAP-Seq Identification of Transcription Factor-Binding Sites in Potato

      • José M. Franco-Zorrilla, Salomé Prat
      Pages 123-142

    6. Mass Spectrometric Monitoring of Plant Hormone Cross Talk During Biotic Stress Responses in Potato (Solanum tuberosum L.)

      • Marta-Marina Pérez-Alonso, Paloma Ortiz-García, José Moya-Cuevas, Stephan Pollmann
      Pages 143-154

    7. A Comprehensive Guide to Potato Transcriptome Assembly

      • Maja Zagorščak, Marko Petek
      Pages 155-192

  4. Bioinformatics and In Silico Approaches

    1. Front Matter

      Pages 193-193
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    2. MapMan Visualization of RNA-Seq Data Using Mercator4 Functional Annotations

      • Marie Bolger, Rainer Schwacke, Björn Usadel
      Pages 195-212

    3. Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing ( dRenSeq )

      • Miles Armstrong, Ingo Hein
      Pages 213-219

    4. Methodologies for Discovery and Quantitative Profiling of sRNAs in Potato

      • Maja Križnik, Maja Zagorščak, Kristina Gruden
      Pages 221-260

    5. Co-expression for Genotype-Phenotype Function Annotation in Potato Research

      • Oren Tzfadia
      Pages 261-272

    6. Computer Vision and Less Complex Image Analyses to Monitor Potato Traits in Fields

      • Junfeng Gao, Jesper Cairo Westergaard, Erik Alexandersson
      Pages 273-299

  5. Molecular Biology Approaches

    1. Front Matter

      Pages 301-301
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    2. Quantifying the Contribution to Virulence of Phytophthora infestans Effectors in Potato

      • Aviv Andriani, Doret Wouters, Pieter J. Wolters, Vivianne G. A. A. Vleeshouwers
      Pages 303-313

    3. Identification of Solanum Immune Receptors by Bulked Segregant RNA-Seq and High-Throughput Recombinant Screening

      • Yerisf Torres Ascurra, Xiao Lin, Pieter J. Wolters, Vivianne G. A. A. Vleeshouwers
      Pages 315-330
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Keywords

About this book

This volume offers a comprehensive collection of experimental approaches to study Solanum tuberosum (potato). Chapters are divided into five sections covering a general overview of potato, the research fields of  molecular biology, omics approaches, and bioinformatics, and finally, the application of the generated knowledge for crop improvement. Written in the format of the highly successful Methods in Molecular Biology series, each wet-lab chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and practical, Solanum tuberosum: Methods and Protocols aims to provide a collection of methods for the potato research community that will enable scientists gain insight into the world of the potato.


Chapter 2 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia

    David Dobnik, Kristina Gruden, Živa Ramšak, Anna Coll

Bibliographic Information

  • Book Title: Solanum tuberosum

  • Book Subtitle: Methods and Protocols

  • Editors: David Dobnik, Kristina Gruden, Živa Ramšak, Anna Coll

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1609-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1608-6Published: 27 August 2021

  • Softcover ISBN: 978-1-0716-1611-6Published: 28 August 2022

  • eBook ISBN: 978-1-0716-1609-3Published: 26 August 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 418

  • Number of Illustrations: 13 b/w illustrations, 92 illustrations in colour

  • Topics: Food Science, Bioinformatics

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