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Stem Cell Renewal and Cell-Cell Communication

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa, Canada

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2346)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Inference of Ligand–Receptor Pairs from Single-Cell Transcriptomics Data

    • Mirjana Efremova, Roser Vento-Tormo
    Pages 1-10

  3. Multiple Imaging Modalities for Cell-Cell Communication via Calcium Mobilizations in Corneal Epithelial Cells

    • Yoonjoo K. Lee, Kristen L. Segars, Vickery Trinkaus-Randall
    Pages 11-20

  4. Interactions of Hematopoietic Stem Cells with Bone Marrow Niche

    • Xinghui Zhao, Cuiping Zhang, Xiaojing Cui, Ying Liang
    Pages 21-34

  5. Ex Vivo Modeling of Hematopoietic Stem Cell Homing to the Fetal Liver

    • Amina Mohammadalipour, Miguel F. Diaz, Sumedha Pareek, Pamela L. Wenzel
    Pages 35-50

  6. Analysis of Epithelial Architecture and Planar Spindle Orientation in the Drosophila Wing Disc

    • Yu-ichiro Nakajima
    Pages 51-62

  7. A Co-culture Model to Study the Effect of Kidney Fibroblast-p90RSK on Epithelial Cell Survival

    • Ling Lin, Samantha White, Kebin Hu
    Pages 63-71

  8. Calcium Fluorescence Recordings from Neuroepithelial Stem Cells

    • Masayuki Yamashita
    Pages 73-78

  9. Ultrastructural Analysis of Cell–Cell Interactions in Drosophila Ovary

    • Matthew Antel, Valentina Baena, Mark Terasaki, Mayu Inaba
    Pages 79-90

  10. TIRF Microscopy as a Tool to Determine Exosome Composition

    • Noa B. Martín-Cófreces, Daniel Torralba, Marta Lozano-Prieto, Nieves Fernández-Gallego, Francisco Sánchez-Madrid
    Pages 91-104

  11. Rapid Visualization of Intracellular Vesicle Events During Synaptic Stimulation

    • Noa B. Martín-Cófreces, Amelia Rojas-Gomez, Sara G. Dosil, Irene Fernandez-Delgado, Francisco Sánchez-Madrid
    Pages 105-120

  12. Monitoring of Active Notch Signaling in Mouse Bladder Urothelium

    • Panagiotis Karakaidos, Theodoros Rampias
    Pages 121-134

  13. Examining Local Cell-to-Cell Signalling in the Kidney Using ATP Biosensing

    • Gareth W. Price, Joe A. Potter, Bethany M. Williams, Chelsy L. Cliff, Mark J. Wall, Claire E. Hills et al.
    Pages 135-149

  14. Isolation and Assessment of Pancreatic Islets Versus Dispersed Beta Cells: A Straightforward Approach to Examine Cell–Cell Communication

    • Rachel T. Scarl, William J. Koch, Kathryn L. Corbin, Craig S. Nunemaker
    Pages 151-164

  15. Promoter Pull-Down Assay: A Biochemical Screen for DNA-Binding Proteins

    • Ryan R. Chaparian, Julia C. van Kessel
    Pages 165-172

  16. Purification of the Vibrio Quorum-Sensing Transcription Factors LuxR, HapR, and SmcR

    • Jane D. Newman, Julia C. van Kessel
    Pages 173-182

  17. Preserving Cytonemes for Immunocytochemistry of Cultured Adherent Cells

    • Sally Rogers, Steffen Scholpp
    Pages 183-190

  18. Fluorescent Labeling of Connexin with As Complex and X-Y Coordinate Registration of Target Single Cells Based on a Triangle Standard Chip for the Image Analysis of Gap Junctional Communication

    • Mikako Saito
    Pages 191-206

  19. Chemical and Voltage Gating of Gap Junction Channels Expressed in Xenopus Oocytes

    • Camillo Peracchia
    Pages 207-214

  20. The Analysis of Gap Junctional Intercellular Communication Among Osteocytes in Chick Calvariae by Fluorescence Recovery After Photobleaching

    • Ziyi Wang, Yoshihito Ishihara, Hiroshi Kamioka
    Pages 215-223
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Keywords

About this book

This detailed book brings together a new set of protocols to arm cell biologists with techniques that are currently being used in a number of well-established laboratories around the world. The contents represent the great strides made in the field of cell-cell communications with respect to the identification and characterization of key components of the communication apparatus, assembly and maintenance of the communications structures, and concomitantly their roles in not only tissue formation and maintenance but also regeneration and repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Stem Cell Renewal and Cell-Cell Communication: Methods and Protocols, Second Edition serves as an ideal guide for experts and newcomers alike seeking to increase our understanding of the crucial biological and physiological roles of cell-cell communications in tissue function and organismal integrity.

Editors and Affiliations

  • Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Stem Cell Renewal and Cell-Cell Communication

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1570-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1569-0Published: 15 May 2021

  • Softcover ISBN: 978-1-0716-1572-0Published: 15 May 2022

  • eBook ISBN: 978-1-0716-1570-6Published: 14 May 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 244

  • Number of Illustrations: 7 b/w illustrations, 43 illustrations in colour

  • Topics: Stem Cells

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