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  • © 2021

Cardiovascular Development

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2319)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
  2. Preparation of Neonatal Rat Papillary Muscles for Contractile Studies

    • Steven Jokerst, Damir Nizmutdinov, Charley Edgar, April M. Kaspick, Carl W. Tong, David E. Dostal
    Pages 31-44
  3. Laser-Induced Choroidal Neovascularization in Rats

    • Min Zhao, Wankun Xie, Travis W. Hein, Lih Kuo, Robert H. Rosa Jr
    Pages 77-85
  4. Detecting Three-Dimensional Vascular Networks in the Mouse Embryonic Hindbrain

    • Chenshen Huang, Liangjing Wu, Jian Wang, Binu Tharakan, Xu Peng
    Pages 87-92
  5. Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy

    • Samantha J. Fredrickson, Tanner G. Hoog, Ryan S. Udan
    Pages 93-104
  6. Visualization of Retinal Blood Vessels

    • Wankun Xie, Min Zhao, Travis W. Hein, Lih Kuo, Robert H. Rosa Jr
    Pages 111-117
  7. Transient Transgenics: An Efficient Method to Identify Gene Regulatory Elements

    • Warren E. Zimmer, Robert J. Schwartz
    Pages 119-136
  8. Isolation of Lymphatic Muscle Cells (LMCs ) from Rat Mesentery

    • Xueyang Zhang, Sanjukta Chakraborty, Mariappan Muthuchamy, David C. Zawieja
    Pages 137-141
  9. Isolation of Adult Mouse Cardiomyocytes Using Langendorff Perfusion Apparatus

    • Yang Liu, David E. Dostal, Carl W. Tong
    Pages 143-152
  10. Analysis of Lymphatic Vessel Formation by Whole-Mount Immunofluorescence Staining

    • Jian Wang, Yuwei Dong, Mariappan Muthuchamy, David C. Zawieja, Xu Peng
    Pages 153-159
  11. Back Matter

    Pages 161-162

About this book

This second edition brings together a variety of method used in the field of cardiovascular development research. Chapters guide readers through protocols on lymphatic development, lymphatic staining techniques, lymphatic endothelial, tissue clearing techniques, and muscle cell isolation and culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls

Authoritative and practical, Cardiovascular Development: Methods and Protocols, Second Edition seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research.

Editors and Affiliations

  • Med. Physiology, Health Science Center, Texas A&M University, Bryan, USA

    Xu Peng, Warren E. Zimmer

Bibliographic Information

  • Book Title: Cardiovascular Development

  • Book Subtitle: Methods and Protocols

  • Editors: Xu Peng, Warren E. Zimmer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1480-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1479-2Published: 31 July 2021

  • Softcover ISBN: 978-1-0716-1482-2Published: 31 July 2022

  • eBook ISBN: 978-1-0716-1480-8Published: 30 July 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 162

  • Number of Illustrations: 4 b/w illustrations, 33 illustrations in colour

  • Topics: Biomedicine, general, Cardiology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access