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Mitochondrial Regulation

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Carlos M. Palmeira

    1. Department of Life Sciences, University of Coimbra, Coimbra, Portugal

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    You can also search for this editor in PubMed   Google Scholar

  2. Anabela P. Rolo

    1. Department of Life Sciences, University of Coimbra, Coimbra, Portugal

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2310)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of miRNA and Protein Expression

    • André L. Simão, Pedro M. Borralho, Clifford J. Steer, Rui E. Castro, Cecília M. P. Rodrigues
    Pages 1-15

  3. Determination of Oxidative Phosphorylation Complexes Activities

    • João S. Teodoro, Ivo F. Machado, Carlos M. Palmeira, Anabela P. Rolo
    Pages 17-31

  4. BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function

    • Elena Silvestri, Assunta Lombardi, Federica Cioffi, Fernando Goglia
    Pages 33-45

  5. Monitoring Mitochondrial Function in Mouse Embryonic Stem Cells (mESCs)

    • Bibiana Correia, Maria Inês Sousa, Ana F. Branco, João Ramalho-Santos
    Pages 47-56

  6. Mitochondrial Functional Assessment in Mammalian Gametes Using Fluorescent Probes

    • Renata S. Tavares, Sara Escada-Rebelo, Maria M. Soares, Andreia F. Silva, Teresa Almeida-Santos, Sandra Amaral et al.
    Pages 57-68

  7. Update on the Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Human Frozen Sections

    • Mariusz R. Wieckowski, Maciej Pronicki, Agnieszka Karkucinska-Wieckowska
    Pages 69-77

  8. An Update on Isolation of Functional Mitochondria from Cells for Bioenergetics Studies

    • Magdalena Lebiedzinska-Arciszewska, Lech Wojtczak, Mariusz R. Wieckowski
    Pages 79-89

  9. PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species

    • Tess C. Leuthner, Jessica H. Hartman, Ian T. Ryde, Joel N. Meyer
    Pages 91-111

  10. Methods to Monitor Mitophagy and Mitochondrial Quality: Implications in Cancer, Neurodegeneration, and Cardiovascular Diseases

    • Simone Patergnani, Massimo Bonora, Esmaa Bouhamida, Alberto Danese, Saverio Marchi, Giampaolo Morciano et al.
    Pages 113-159

  11. Analysis of Proapoptotic Protein Trafficking to and from Mitochondria

    • Ignacio Vega-Naredo, Gabriela Oliveira, Teresa Cunha-Oliveira, Teresa Serafim, Vilma A. Sardão, Paulo J. Oliveira
    Pages 161-178

  12. Exploring Liver Mitochondrial Function by 13C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry

    • Emilio Molina-Molina, Harshitha Shanmugam, Domenica Di Palo, Ignazio Grattagliano, Piero Portincasa
    Pages 179-199

  13. Protocols for Mitochondria as the Target of Pharmacological Therapy in the Context of Nonalcoholic Fatty Liver Disease (NAFLD)

    • Ignazio Grattagliano, Agostino Di Ciaula, Jacek Baj, Emilio Molina-Molina, Harshitha Shanmugam, Gabriella Garruti et al.
    Pages 201-246

  14. The Use of Reactive Oxygen Species Production by Succinate-Driven Reverse Electron Flow as an Index of Complex 1 Activity in Isolated Brown Adipose Tissue Mitochondria

    • Andrea Dlasková, Kieran J. Clarke, Mary F. Rooney, Richard K. Porter
    Pages 247-258

  15. Mitochondrial Regulation Assessment by 13C-NMR Isotopomer Analysis

    • Francisco X. Carvalho, Bárbara Guerra-Carvalho, Ivana Jarak, Rui A. Carvalho
    Pages 259-270

  16. Assays for Determination of Cellular and Mitochondrial NAD+ and NADH Content

    • Yue Yang, Anthony A. Sauve
    Pages 271-285

  17. Following the Dynamism of the Mitochondrial Network in T Cells

    • Arianna Di Daniele, Luca Simula, Silvia Campello
    Pages 287-299

  18. Measuring PGC-1α and Its Acetylation Status in Mouse Primary Myotubes

    • João A. Amorim, David A. Sinclair
    Pages 301-309

  19. Back Matter

    Pages 311-318
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Keywords

About this book

This fully updated edition explores the different pathways that converge into the regulation of mitochondrial function. The book integrates mitochondria with other cellular components, discussing the dynamic properties of mitochondria with an emphasis on how these processes respond to signaling events and how they affect cellular metabolism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Mitochondrial Regulation: Methods and Protocols, Second Edition is an ideal guide for advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry, and bioenergetics.

Editors and Affiliations

  • Department of Life Sciences, University of Coimbra, Coimbra, Portugal

    Carlos M. Palmeira, Anabela P. Rolo

Bibliographic Information

  • Book Title: Mitochondrial Regulation

  • Book Subtitle: Methods and Protocols

  • Editors: Carlos M. Palmeira, Anabela P. Rolo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1433-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1432-7Published: 08 June 2021

  • Softcover ISBN: 978-1-0716-1435-8Published: 07 June 2021

  • eBook ISBN: 978-1-0716-1433-4Published: 07 June 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 318

  • Number of Illustrations: 17 b/w illustrations, 47 illustrations in colour

  • Topics: Cell Biology, Biological and Medical Physics, Biophysics

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