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Methods in Molecular Biology

Yarrowia lipolytica

Methods and Protocols

Editors: Wheeldon, Ian, Blenner, Mark (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $139.00
price for USA in USD
  • ISBN 978-1-0716-1414-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-0716-1413-6
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
About this book

This volume provides a collection of protocols for the most common experimental methods used for engineering Yarrowia lipolytica. Chapters detail the basic theories underlying the methods described in each chapter. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Yarrowia lipolytica: Methods and Protocols aims to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully.


Table of contents (16 chapters)

Table of contents (16 chapters)
  • Genome Engineering of Yarrowia lipolytica with the PiggyBac Transposon System

    Pages 1-24

    Wagner, James M. (et al.)

  • Genome Editing in Y. lipolytica Using TALENs

    Pages 25-39

    Rigouin, Coraline (et al.)

  • EasyCloneYALI: Toolbox for CRISPR-Mediated Integrations and Deletions in Yarrowia lipolytica

    Pages 41-68

    Dahlin, Jonathan (et al.)

  • Simultaneous Gene Excision and Integration by Dual-Guide CRISPR-Cas9

    Pages 69-83

    Spagnuolo, Michael (et al.)

  • Gene Excision by Dual-Guide CRISPR-Cas9

    Pages 85-94

    Spagnuolo, Michael (et al.)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-0716-1414-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-0716-1413-6
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Yarrowia lipolytica
Book Subtitle
Methods and Protocols
Editors
  • Ian Wheeldon
  • Mark Blenner
Series Title
Methods in Molecular Biology
Series Volume
2307
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-1414-3
DOI
10.1007/978-1-0716-1414-3
Hardcover ISBN
978-1-0716-1413-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 250
Number of Illustrations
21 b/w illustrations, 44 illustrations in colour
Topics