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Methods in Molecular Biology
cover

RNA Modifications

Methods and Protocols

Editors: McMahon, Mary (Ed.)

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $169.00
price for USA in USD
  • ISBN 978-1-0716-1374-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-0716-1373-3
  • with online files
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
About this book

This detailed book describes some of the most recent advances and up-to-date methodologies to detect, quantify, analyze, and elucidate the biological function of different types of RNA modifications. Importantly, the methodologies and tools described herein can be applied to a wide variety of organisms and can be used to address biological and clinical questions. Beginning with a section on bioinformatics tools, the collection continues with sections on detecting RNA modifications using Nanopore direct RNA sequencing, next-generation sequencing approaches, qPCR- and molecular biology-based methods, mass spectrometry- and NMR-based methods, as well as approaches to assess kinetics, determinants, and functions of RNA modifications. Written for the highly successful Methods in Molecular Biology series style, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, RNA Modifications: Methods and Protocols serves as an ideal guide for those working directly in the fields of epitranscriptomics and post-transcriptional gene regulation, as well as for scientists and clinicians interested in bioinformatic tools to study RNA modifications and techniques to dissect their roles in physiology and disease.
Chapter 20 is available open access under a CC BY 4.0 license.

Table of contents (23 chapters)

Table of contents (23 chapters)
  • RNA Post-Transcriptional Modification Mapping Data Analysis Using RNA Framework

    Pages 3-13

    Manfredonia, Ilaria (et al.)

  • An Informatics Pipeline for Profiling and Annotating RNA Modifications

    Pages 15-27

    Liu, Qi (et al.)

  • EpiNano: Detection of m6A RNA Modifications Using Oxford Nanopore Direct RNA Sequencing

    Pages 31-52

    Liu, Huanle (et al.)

  • Adaptation of Human Ribosomal RNA for Nanopore Sequencing of Canonical and Modified Nucleotides

    Pages 53-74

    Jain, Miten (et al.)

  • AlkAniline-Seq: A Highly Sensitive and Specific Method for Simultaneous Mapping of 7-Methyl-guanosine (m7G) and 3-Methyl-cytosine (m3C) in RNAs by High-Throughput Sequencing

    Pages 77-95

    Marchand, Virginie (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-0716-1374-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-0716-1373-3
  • with online files
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
RNA Modifications
Book Subtitle
Methods and Protocols
Editors
  • Mary McMahon
Series Title
Methods in Molecular Biology
Series Volume
2298
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-1374-0
DOI
10.1007/978-1-0716-1374-0
Hardcover ISBN
978-1-0716-1373-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 427
Number of Illustrations
2 b/w illustrations, 72 illustrations in colour
Topics