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Urinary Biomarkers

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Samanta Salvi

    1. Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy

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  2. Valentina Casadio

    1. Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2292)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
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  2. Urinary Biomarkers in Cancer

    1. Front Matter

      Pages 1-1
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    2. Urinary Biomarkers in Tumors: An Overview

      • Ilaria Cimmino, Sara Bravaccini, Claudio Cerchione
      Pages 3-15

    3. Urinary Cell-Free DNA Integrity Analysis

      • Valentina Casadio, Samanta Salvi
      Pages 17-22

    4. Mutational Analysis in Urinary Cell-Free DNA: KRAS in Colorectal Cancer

      • Chiara Molinari, Elisa Chiadini
      Pages 23-33

    5. Fluorescence In Situ Hybridization in Urine Samples (UroVysion Kit)

      • Sara Bravaccini
      Pages 35-48

    6. Analysis of Copy Number Variation in Urine: c-Myc Evaluation Using a Real-Time PCR Approach

      • Valentina Casadio, Filippo Martignano, Roberta Gunelli, Samanta Salvi
      Pages 49-56

    7. Urinary microRNA and mRNA in Tumors

      • Erika Bandini
      Pages 57-72

    8. Long Noncoding RNAs as Innovative Urinary Diagnostic Biomarkers

      • Giulia Brisotto, Roberto Guerrieri, Francesca Colizzi, Agostino Steffan, Barbara Montico, Elisabetta Fratta
      Pages 73-94

    9. Urinary Nucleic Acid in Tumor: Bioinformatics Approaches

      • Davide Angeli
      Pages 95-104

    10. PCA3 in Prostate Cancer

      • Roberta Gunelli, Eugenia Fragalà, Massimo Fiori
      Pages 105-113

    11. Urinary Exosomes in Prostate Cancer

      • Samanta Salvi, Erika Bandini, Francesco Fabbri
      Pages 115-120

    12. Urinary Biomarkers In Bladder Cancer

      • Matteo Costantini, Graziana Gallo, Giovanna Attolini
      Pages 121-131

    13. Telomerase Activity Analysis In Urine Sediment for Bladder Cancer

      • Valentina Casadio, Sara Bravaccini
      Pages 133-141

    14. Protocols for Preparation and Mass Spectrometry Analysis of Clinical Urine Samples to Identify Candidate Biomarkers of Schistosoma-Associated Bladder Cancer

      • Tariq Ganief, Bridget Calder, Jonathan M. Blackburn
      Pages 143-150

  3. Urinary Extracellular Vesicles

    1. Front Matter

      Pages 151-151
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    2. Current Methods for the Isolation of Urinary Extracellular Vesicles

      • Serena Maggio, Emanuela Polidori, Paola Ceccaroli, Andrea Cioccoloni, Vilberto Stocchi, Michele Guescini
      Pages 153-172

    3. Urinary Extracellular Vesicles: Ultracentrifugation Method

      • Eisuke Tomiyama, Kazutoshi Fujita, Norio Nonomura
      Pages 173-181

  4. Physical Activity and Urinary Markers

    1. Front Matter

      Pages 183-183
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    2. Urinary Catecholamines as Markers in Overtraining Syndrome

      • Marina Casadio
      Pages 185-192
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Keywords

About this book

This volume describes important methods, protocols, and techniques used for studying urinary biomarkers. Chapters detail different alterations use to studied different types of cancers and physiological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Urinary Biomarkers: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.


Editors and Affiliations

  • Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy

    Samanta Salvi, Valentina Casadio

Bibliographic Information

  • Book Title: Urinary Biomarkers

  • Book Subtitle: Methods and Protocols

  • Editors: Samanta Salvi, Valentina Casadio

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1354-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1353-5Published: 03 March 2021

  • Softcover ISBN: 978-1-0716-1356-6Published: 04 March 2022

  • eBook ISBN: 978-1-0716-1354-2Published: 02 March 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 214

  • Number of Illustrations: 3 b/w illustrations, 14 illustrations in colour

  • Topics: Molecular Medicine

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