Methods in Molecular Biology

Design and Delivery of SiRNA Therapeutics

Editors: Ditzel, Henrik J., Tuttolomondo, Martina, Kauppinen, Sakari (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $169.00
price for USA in USD
  • ISBN 978-1-0716-1298-9
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Hardcover $219.99
price for USA in USD
  • ISBN 978-1-0716-1297-2
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About this book

This volume details protocols on rationale design of therapeutic siRNA molecules and its encapsulation with smart vehicles to overcome the barriers to an effective administration in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Design and Delivery of SiRNA Therapeutics aims to ensure successful results in the further study of this vital field.

Table of contents (24 chapters)

Table of contents (24 chapters)
  • RNA Interference: Story and Mechanisms

    Pages 1-15

    Sioud, Mouldy

  • Selection of Chemical Modifications in the siRNA Seed Region That Repress Off-Target Effect

    Pages 17-30

    Kobayashi, Yoshiaki (et al.)

  • Stick-Based Methods for Aptamer-Mediated siRNA Targeted Delivery

    Pages 31-42

    Catuogno, Silvia (et al.)

  • Evaluation of siRNA Stability and Interaction with Serum Components Using an Agarose Gel-Based Single-Molecule FRET Labeling Method

    Pages 43-56

    Tuttolomondo, Martina (et al.)

  • Development of siRNA Therapeutics for the Treatment of Liver Diseases

    Pages 57-75

    Holm, Anja (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-0716-1298-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-0716-1297-2
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Design and Delivery of SiRNA Therapeutics
Editors
  • Henrik J. Ditzel
  • Martina Tuttolomondo
  • Sakari Kauppinen
Series Title
Methods in Molecular Biology
Series Volume
2282
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-1298-9
DOI
10.1007/978-1-0716-1298-9
Hardcover ISBN
978-1-0716-1297-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 469
Number of Illustrations
44 b/w illustrations, 103 illustrations in colour
Topics