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Design and Delivery of SiRNA Therapeutics

  • Book
  • © 2021

Overview

Editors:
  1. Henrik J. Ditzel

    1. Department of Oncology, Odense University Hospital Odense, Denmark, Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark

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  2. Martina Tuttolomondo

    1. Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark

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  3. Sakari Kauppinen

    1. Center for RNA Medicine, Department of Clinical Medicine, Aalborg University, Copenhagen, Denmark

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2282)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. RNA Interference: Story and Mechanisms

    • Mouldy Sioud
    Pages 1-15

  3. Selection of Chemical Modifications in the siRNA Seed Region That Repress Off-Target Effect

    • Yoshiaki Kobayashi, Kengo Miyamoto, Misako Aida, Kumiko Ui-Tei
    Pages 17-30

  4. Stick-Based Methods for Aptamer-Mediated siRNA Targeted Delivery

    • Silvia Catuogno, Carla Lucia Esposito, Paloma H. Giangrande
    Pages 31-42

  5. Evaluation of siRNA Stability and Interaction with Serum Components Using an Agarose Gel-Based Single-Molecule FRET Labeling Method

    • Martina Tuttolomondo, Henrik J. Ditzel
    Pages 43-56

  6. Development of siRNA Therapeutics for the Treatment of Liver Diseases

    • Anja Holm, Marianne Bengtson Løvendorf, Sakari Kauppinen
    Pages 57-75

  7. siRNA Design and GalNAc-Empowered Hepatic Targeted Delivery

    • Mei Lu, Mengjie Zhang, Bo Hu, Yuanyu Huang
    Pages 77-100

  8. Synthesis of GalNAc-Oligonucleotide Conjugates Using GalNAc Phosphoramidite and Triple-GalNAc CPG Solid Support

    • Egor A. Ulashchik, Yury V. Martynenko-Makaev, Tatsiana P. Akhlamionok, Denis M. Melnik, Vadim V. Shmanai, Timofei S. Zatsepin
    Pages 101-118

  9. Preparation of Lipid-Conjugated siRNA Oligonucleotides for Enhanced Gene Inhibition in Mammalian Cells

    • Santiago Grijalvo, Sandra M. Ocampo, José Carlos Perales, Ramon Eritja
    Pages 119-136

  10. Engineering of Solid Dosage Forms of siRNA-Loaded Lipidoid–Polymer Hybrid Nanoparticles Using a Quality-by-Design Approach

    • Abhijeet Lokras, Camilla Foged, Aneesh Thakur
    Pages 137-157

  11. Preparation and Characterization of siRNA-Loaded Liposomes

    • Yang Liu, Leaf Huang
    Pages 159-169

  12. siRNA Vehicles for High Endosomal Escapability

    • Hiroyuki Koide, Sei Yonezawa, Tomohiro Asai
    Pages 171-179

  13. siRNA Design and Delivery Based on Carbon Nanotubes

    • Danyang Li, Khuloud T. Al-Jamal
    Pages 181-193

  14. MRI-Visible Nanovehicle for Efficient siRNA Delivery

    • Rongrong Jin, Wencheng Zhu, Gan Lin, Gang Liu, Hua Ai
    Pages 195-208

  15. Cationic Dendrimers for siRNA Delivery: An Overview of Methods for In Vitro/In Vivo Characterization

    • Erik Laurini, Suzana Aulic, Domenico Marson, Maurizio Fermeglia, Sabrina Pricl
    Pages 209-244

  16. ITC for Characterization of Self-Assembly Process of Cationic Dendrons for siRNA Delivery

    • Erik Laurini, Suzana Aulic, Natasa Skoko, Domenico Marson, Maurizio Fermeglia, Sabrina Pricl
    Pages 245-266

  17. Cationic Dendrimers for siRNA Delivery: Computational Approaches for Characterization

    • Domenico Marson, Suzana Aulic, Maurizio Fermeglia, Erik Laurini, Sabrina Pricl
    Pages 267-296

  18. Fundamental and Practical Aspects in the Formulation of Colloidal Polyelectrolyte Complexes of Chitosan and siRNA

    • Christophe Schatz, Tim Delas
    Pages 297-327

  19. Cell-Penetrating Peptides Delivering siRNAs: An Overview

    • Luca Falato, Maxime Gestin, Ülo Langel
    Pages 329-352

  20. Non-covalent Encapsulation of siRNA with Cell-Penetrating Peptides

    • Martina Tuttolomondo, Henrik J. Ditzel
    Pages 353-376
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Keywords

About this book

This volume details protocols on rationale design of therapeutic siRNA molecules and its encapsulation with smart vehicles to overcome the barriers to an effective administration in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Design and Delivery of SiRNA Therapeutics aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Oncology, Odense University Hospital Odense, Denmark, Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark

    Henrik J. Ditzel

  • Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark

    Martina Tuttolomondo

  • Center for RNA Medicine, Department of Clinical Medicine, Aalborg University, Copenhagen, Denmark

    Sakari Kauppinen

Bibliographic Information

  • Book Title: Design and Delivery of SiRNA Therapeutics

  • Editors: Henrik J. Ditzel, Martina Tuttolomondo, Sakari Kauppinen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1298-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1297-2Published: 30 April 2021

  • Softcover ISBN: 978-1-0716-1300-9Published: 30 April 2022

  • eBook ISBN: 978-1-0716-1298-9Published: 30 April 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 469

  • Number of Illustrations: 44 b/w illustrations, 103 illustrations in colour

  • Topics: Biomedicine, general, Genetics and Genomics

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