Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2272)
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Table of contents (18 protocols)
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Front Matter
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Detection and Quantification of DNA Methylation Turnover
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Front Matter
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Bioinformatic Analysis of DNA Methylation and Hydroxymethylation
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Front Matter
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Precise Epigenome Manipulation Using TET Enzymes
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Front Matter
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TET and DNA Hydroxymethylation Biochemistry
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Front Matter
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About this book
This volume explores the latest methods used to study various aspects of TET proteins and their biology. Chapters in this book are divided into five parts. Part One describes technologies aimed at detecting and quantifying DNA methylation turnover using massively parallel sequencing, ELISA, and mass spectrometry approaches. Part Two looks at data analyses protocols for distinguishing acting versus passive DNA demethylation and estimation of 5mC and 5hmC levels. Part Three deals with a new topic that takes advantage of modified CRISPR/Cas9 genome editing systems to target DNA demethylation activity to genomic loci of interest. Part Four discusses protocols that detail how to purify TET proteins and unravel their protein interactions, and Part Five looks at the assessment of TET protein function and activity in vivo and in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, TET Proteins and DNA Demethylation: Methods and Protocols is a valuable resource that aims to help research scientists at all levels working in the fields of DNA demethylation dynamics.
Chapters 3, 7 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Editors and Affiliations
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Genomics and Epigenetics Division, Garvan Institute of Medical Research, Darlinghurst, Australia
Ozren Bogdanovic
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Radboud University, Nijmegen, The Netherlands
Michiel Vermeulen
Bibliographic Information
Book Title: TET Proteins and DNA Demethylation
Book Subtitle: Methods and Protocols
Editors: Ozren Bogdanovic, Michiel Vermeulen
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1294-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021
Hardcover ISBN: 978-1-0716-1293-4Published: 20 May 2021
Softcover ISBN: 978-1-0716-1296-5Published: 20 May 2021
eBook ISBN: 978-1-0716-1294-1Published: 19 May 2021
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 320
Number of Illustrations: 10 b/w illustrations, 46 illustrations in colour
Topics: Protein Science, Human Genetics