Springer Protocols Handbooks

DNA Modification Detection Methods

Editors: Yuan, Bi-Feng (Ed.)

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  • Provides a comprehensive overview of available techniques and methods for studying various types of DNA modifications
  • Organized as detailed step-by-step protocols for experimental procedures required to successfully perform analysis
  • Offers new opportunities for researchers to manipulate the modification status to affect gene expression, and develop small molecules to tune the DNA functions
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  • ISBN 978-1-0716-1229-3
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About this book

Understanding the functional roles of DNA modifications relies on the accurate detection, quantification, and mapping of DNA modifications. Methods for deciphering DNA modifications have substantially improved over the last several years, which greatly revolutionize the field of DNA modifications. In addition to DNA cytosine methylation (5-methylcytosine, 5mC), the best-characterized epigenetic modification, many new modifications have been discovered to present in DNA in recent years.  

This book provides a comprehensive overview of available techniques and methods together with detailed step-by-step protocols for experimental procedures required to successfully perform analysis on various types of DNA modifications, including 5mC, 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxycytosine (5caC), 5-hydroxymethyluracil (5hmU), 5-formyluracil (5fU), N6-methyladenine (6mA), β-D-glucosyl-5-hydroxymethyluracil (base J) and 8-oxo-7,8-dihydroguanine (OG). This laboratory manual is a valuable source for biochemists and molecular biologists from different fields who wish to investigate DNA modifications.


About the authors

Dr. Yuan received his bachelor’s degree and PhD degree from Wuhan University in 2001 and 2006, respectively. After completing the postdoctoral research in National University of Singapore (2006-2007, Singapore) and University of California, Riverside (2007-2010, USA), Dr. Yuan started to work as a professor in the College of Chemistry and Molecular Sciences of Wuhan University from 2011. Dr. Yuan’s research focuses on the development and application of new analytical techniques in the investigation of the occurrence, location, and biological functions of nucleic acid modifications. By establishing highly sensitive analytical methods, Dr. Yuan discovered many new types of modifications in nucleic acids. He also revealed the mechanisms of the formation and metabolism of some nucleic acid modifications, which promotes the understanding of the biological functions of nucleic acid modifications.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Quantitative Assessment of the Oxidation Products of 5-Methylcytosine in DNA by Liquid Chromatography-Tandem Mass Spectrometry

    Pages 3-11

    Yin, Jiekai (et al.)

  • Determination of Cytosine Modifications in DNA by Chemical Labeling-Mass Spectrometry Analysis

    Pages 13-21

    Cheng, Qing-Yun (et al.)

  • Analysis of 5-Methylcytosine and 5-Hydroxymethylcytosine in Genomic DNA by Capillary Electrophoresis-Mass Spectrometry

    Pages 23-30

    Yuan, Fang (et al.)

  • Simple Quantification of Epigenetic DNA Modifications and DNA Damage on Multi-Well Slides

    Pages 31-44

    Michaeli, Yael (et al.)

  • A Label-Free Electrochemical Biosensor for Sensitive Detection of 5-Hydroxymethylcytosine

    Pages 45-52

    Cui, Lin (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-0716-1229-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-0716-1228-6
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
DNA Modification Detection Methods
Editors
  • Bi-Feng Yuan
Series Title
Springer Protocols Handbooks
Copyright
2022
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-1229-3
DOI
10.1007/978-1-0716-1229-3
Hardcover ISBN
978-1-0716-1228-6
Series ISSN
1949-2448
Edition Number
1
Number of Pages
VI, 194
Number of Illustrations
18 b/w illustrations, 30 illustrations in colour
Topics