Methods in Molecular Biology

Ras Activity and Signaling

Methods and Protocols

Editors: Rubio, Ignacio, Prior, Ian (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $169.00
price for USA in USD
  • ISBN 978-1-0716-1190-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-0716-1189-0
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About this book

This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output. Chapters detail standard methodologies, biochemical methods, Ras processing trafficking and localization, Ras signaling and inhibition, and in vivo models for studying Ras function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Ras Activity and Signaling: Methods and Protocols aims to provide support and guidance to lab workers in their work on Ras GTPases and in the design of new projects requiring novel methodologies.

Table of contents (26 chapters)

Table of contents (26 chapters)
  • Ras Variant Biology and Contributions to Human Disease

    Pages 3-18

    Prior, Ian

  • Regulation of the Small GTPase Ras and Its Relevance to Human Disease

    Pages 19-43

    Kulhanek, Kayla R. (et al.)

  • Precise Characterization of KRAS4B Proteoforms by Combining Immunoprecipitation with Top-Down Mass Spectrometry

    Pages 47-64

    Adams, Lauren M. (et al.)

  • Absolute Quantitation of GTPase Protein Abundance

    Pages 65-90

    Hood, Fiona E. (et al.)

  • Validation of Isoform- and Mutation-Specific RAS Antibodies

    Pages 91-103

    Waters, Andrew M. (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-0716-1190-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-0716-1189-0
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Ras Activity and Signaling
Book Subtitle
Methods and Protocols
Editors
  • Ignacio Rubio
  • Ian Prior
Series Title
Methods in Molecular Biology
Series Volume
2262
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-1190-6
DOI
10.1007/978-1-0716-1190-6
Hardcover ISBN
978-1-0716-1189-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 439
Number of Illustrations
29 b/w illustrations, 57 illustrations in colour
Topics