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Methods in Molecular Biology

Proteomic Profiling

Methods and Protocols

Editors: Posch, Anton (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $219.00
price for USA in USD
  • ISBN 978-1-0716-1186-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $279.99
price for USA in USD
About this book

This detailed new edition presents the latest developments of the main pillars of protein analysis, namely sample preparation, separation, and characterization. Core areas in this volume are protocols for the analysis of post-translational modifications and protein interaction partners, followed by sophisticated procedures to enrich for extracellular vesicles and organelles, along with several types of protein immuno-assays complemented by various methods for the characterization of antibodies and host-cell protein analysis. Last but not least, a few standard sample preparation protocols and recent advances concerning immuno-chemical detection of proteins are included as well. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and up-to-date, Proteomic Profiling: Methods and Protocols, Second Edition serves as an ideal reference for students of biochemistry, biomedicine, biology, and genomics and will be an invaluable source for the experienced, practicing scientist as well.

Table of contents (36 chapters)

Table of contents (36 chapters)
  • How Modern Mass Spectrometry Can Solve Ancient Questions: A Multi-Omics Study of the Stomach Content of the Oldest Human Ice Mummy, the 5300-Year-Old Iceman or Oetzi

    Pages 1-12

    Grimm, Rudolf

  • Step-by-Step Sample Preparation of Proteins for Mass Spectrometric Analysis

    Pages 13-23

    Li, Xinping (et al.)

  • Lab-on-a-Filter Techniques for Economical, Effective, and Flexible Proteome Analysis

    Pages 25-34

    Lin, Yi-Han (et al.)

  • Released N-Glycan Analysis for Biotherapeutic Development Using Liquid Chromatography and Mass Spectrometry

    Pages 35-53

    Zhang, Ximo

  • Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation, and N-Linked Sialylated Glycosylation

    Pages 55-72

    Melo-Braga, Marcella Nunes (et al.)

Buy this book

eBook $219.00
price for USA in USD
  • ISBN 978-1-0716-1186-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $279.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Proteomic Profiling
Book Subtitle
Methods and Protocols
Editors
  • Anton Posch
Series Title
Methods in Molecular Biology
Series Volume
2261
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-1186-9
DOI
10.1007/978-1-0716-1186-9
Hardcover ISBN
978-1-0716-1185-2
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XV, 592
Number of Illustrations
40 b/w illustrations, 91 illustrations in colour
Topics