- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Buy this book
- About this book
-
This detailed new edition presents the latest developments of the main pillars of protein analysis, namely sample preparation, separation, and characterization. Core areas in this volume are protocols for the analysis of post-translational modifications and protein interaction partners, followed by sophisticated procedures to enrich for extracellular vesicles and organelles, along with several types of protein immuno-assays complemented by various methods for the characterization of antibodies and host-cell protein analysis. Last but not least, a few standard sample preparation protocols and recent advances concerning immuno-chemical detection of proteins are included as well. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Proteomic Profiling: Methods and Protocols, Second Edition serves as an ideal reference for students of biochemistry, biomedicine, biology, and genomics and will be an invaluable source for the experienced, practicing scientist as well.
- Table of contents (36 chapters)
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How Modern Mass Spectrometry Can Solve Ancient Questions: A Multi-Omics Study of the Stomach Content of the Oldest Human Ice Mummy, the 5300-Year-Old Iceman or Oetzi
Pages 1-12
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Step-by-Step Sample Preparation of Proteins for Mass Spectrometric Analysis
Pages 13-23
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Lab-on-a-Filter Techniques for Economical, Effective, and Flexible Proteome Analysis
Pages 25-34
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Released N-Glycan Analysis for Biotherapeutic Development Using Liquid Chromatography and Mass Spectrometry
Pages 35-53
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Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation, and N-Linked Sialylated Glycosylation
Pages 55-72
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Table of contents (36 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Proteomic Profiling
- Book Subtitle
- Methods and Protocols
- Editors
-
- Anton Posch
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2261
- Copyright
- 2021
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-1186-9
- DOI
- 10.1007/978-1-0716-1186-9
- Hardcover ISBN
- 978-1-0716-1185-2
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XV, 592
- Number of Illustrations
- 40 b/w illustrations, 91 illustrations in colour
- Topics
