Methods in Molecular Biology

Shotgun Proteomics

Methods and Protocols

Editors: Carrera, Mónica, Mateos, Jesús (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-0716-1178-4
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Hardcover $199.99
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  • ISBN 978-1-0716-1177-7
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About this book

This edition details a collection of specific shotgun proteomics-based laboratory techniques and applications developed in leading laboratories and proteomics units worldwide. Chapters cover a broad range of topics covering, shotgun proteomics of extracellular vesicles and subcellular structures, shotgun proteomics in non-model organisms, clinical proteomics, food proteomics, analysis of post-translational modifications and protein complexes, and data processing and storage. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Shotgun Proteomics: Methods and Protocols aims to be an up-to-date guide for researchers seeking to understand the proteome of any given biological sample.


Table of contents (20 chapters)

Table of contents (20 chapters)
  • Mesenchymal Stem Cell-Derived Extracellular Vesicle Isolation and Their Protein Cargo Characterization

    Pages 3-12

    Morente-López, Miriam (et al.)

  • Clinical Proteomics for the Analysis of Circulating Extracellular Vesicles

    Pages 13-23

    Barrachina, Maria N. (et al.)

  • Utilization of Laser Capture Microdissection Coupled to Mass Spectrometry to Uncover the Proteome of Cellular Protrusions

    Pages 25-45

    Gordon, Ana (et al.)

  • Isolation of Apoplastic Fluid from Woody Plant Leaves: Grapevine and Coffee as a Case Study

    Pages 49-57

    Figueiredo, Andreia (et al.)

  • Shotgun Proteomics for L3 and L4 Anisakis simplex Development Stages

    Pages 59-75

    Stryiński, Robert (et al.)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-0716-1178-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-0716-1177-7
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Shotgun Proteomics
Book Subtitle
Methods and Protocols
Editors
  • Mónica Carrera
  • Jesús Mateos
Series Title
Methods in Molecular Biology
Series Volume
2259
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-1178-4
DOI
10.1007/978-1-0716-1178-4
Hardcover ISBN
978-1-0716-1177-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVII, 324
Number of Illustrations
10 b/w illustrations, 49 illustrations in colour
Topics