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Programmed Morphogenesis

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Mo R. Ebrahimkhani

    1. Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh Liver Research Center, University of Pittsburgh, Department of Bioengineering Swanson School of Engineering, University of Pittsburgh, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, USA

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  2. Joshua Hislop

    1. Department of Bioengineering Swanson School of Engineering, Pittsburgh Liver Research Center, Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2258)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Understanding Signaling Events and Patterns

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. TASBE Image Analytics : A Processing Pipeline for Quantifying Cell Organization from Fluorescent Microscopy

      • Nicholas Walczak, Jacob Beal, Jesse Tordoff, Ron Weiss
      Pages 3-15

    3. Neighborhood Impact Factor to Study Cell-Fate Decision-Making in Cellular Communities

      • Shaylina R. Carter, Joshua Hislop, Joshua Hsu, Jeremy J. Velazquez, Mo R. Ebrahimkhani
      Pages 17-28

    4. A Quantitative Lineage-Tracing Approach to Understand Morphogenesis in Gut

      • Svetlana Ulyanchenko, Jordi Guiu
      Pages 29-40

  3. Programming Signaling Events and Patterns

    1. Front Matter

      Pages 41-41
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    2. Reconstitution of Morphogen Signaling Gradients in Cultured Cells

      • Julia S. Kim, Michael Pineda, Pulin Li
      Pages 43-56

    3. Engineering Shape-Controlled Microtissues on Compliant Hydrogels with Tunable Rigidity and Extracellular Matrix Ligands

      • Megan L. Rexius-Hall, Nethika R. Ariyasinghe, Megan L. McCain
      Pages 57-72

    4. Engineering Biophysical Cues for Controlled 3D Differentiation of Endoderm Derivatives

      • Thomas Richardson, Shibin Mathew, Connor Wiegand, Kevin Pietz, Joseph Candiello, K. Ravikumar et al.
      Pages 73-92

    5. Rewiring Endogenous Bioelectric Circuits in the Xenopus laevis Embryo Model

      • Vasilios Nanos, Michael Levin
      Pages 93-103

    6. Engineering the Spatiotemporal Mosaic Self-Patterning of Pluripotent Stem Cells

      • Ashley R. G. Libby, David A. Joy, Todd C. McDevitt
      Pages 105-116

  4. Early Developmental Engineering

    1. Front Matter

      Pages 117-117
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    2. Fate-Patterning of 2D Gastruloids and Ectodermal Colonies Using Micropatterned Human Pluripotent Stem Cells

      • George Britton, Sapna Chhabra, Joseph Massey, Aryeh Warmflash
      Pages 119-130

    3. Gastruloids: Embryonic Organoids from Mouse Embryonic Stem Cells to Study Patterning and Development in Early Mammalian Embryos

      • Kerim Anlas, Peter Baillie-Benson, Krisztina Arató, David A. Turner, Vikas Trivedi
      Pages 131-147

  5. Organoids, Tissue Barriers, and Disease Models

    1. Front Matter

      Pages 149-149
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    2. A Synergistic Engineering Approach to Build Human Brain Spheroids

      • Djuna von Maydell, Mehdi Jorfi
      Pages 151-169

    3. Directed Differentiation of Human Pluripotent Stem Cells for the Generation of High-Order Kidney Organoids

      • Idoia Lucía Selfa, Maria Gallo, Nuria Montserrat, Elena Garreta
      Pages 171-192

    4. Methods for Controlled Induction of Singular Rosette Cytoarchitecture Within Human Pluripotent Stem Cell-Derived Neural Multicellular Assemblies

      • Alireza Aghayee, Randolph Ashton
      Pages 193-203

    5. 3D Self-Organized Human Blood–Brain Barrier in a Microfluidic Chip

      • Marco Campisi, Sei Hien Lim, Valeria Chiono, Roger Dale Kamm
      Pages 205-219

    6. Modeling the Complexity of the Metastatic Niche Ex Vivo

      • Amanda M. Clark
      Pages 221-239
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Keywords

About this book

This detailed book explores techniques for understanding and engineering programs that naturally control and drive formation of tissues and organs in order to open powerful opportunities to produce physiologically relevant tissues of interest, generate models to study human disease, and set the path for the manufacturing of advanced tissue and organs. Beginning with chapters to help understand signaling events and patterns in morphogenetic systems, the volume continues by covering programming signaling events and patterns to drive morphogenesis, techniques for engineering organoids, tissue barriers, and disease models, as well as in vivo therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Programmed Morphogenesis: Methods and Protocols aims not only to communicate knowledge but also to inspire approaches to new challenges and to empower readers with the capability to approach those challenges.

Editors and Affiliations

  • Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh Liver Research Center, University of Pittsburgh, Department of Bioengineering Swanson School of Engineering, University of Pittsburgh, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, USA

    Mo R. Ebrahimkhani

  • Department of Bioengineering Swanson School of Engineering, Pittsburgh Liver Research Center, Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, USA

    Joshua Hislop

Bibliographic Information

  • Book Title: Programmed Morphogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Mo R. Ebrahimkhani, Joshua Hislop

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1174-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1173-9Published: 20 December 2020

  • Softcover ISBN: 978-1-0716-1176-0Published: 20 December 2021

  • eBook ISBN: 978-1-0716-1174-6Published: 19 December 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 286

  • Number of Illustrations: 1 b/w illustrations, 69 illustrations in colour

  • Topics: Developmental Biology, Biotechnology

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