Skip to main content
SpringerLink
Log in

The TNF Superfamily

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Jagadeesh Bayry

    1. INSERM, Sorbonne Université, Paris, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2248)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 149.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Signaling in TNFSF15-mediated Suppression of VEGF Production in Endothelial Cells

    • Huanyu Zhao, Qiangzhe Zhang
    Pages 1-18

  3. Sensitization of Airway Epithelial Cells to Toxin-Induced Death by TNF Superfamily Cytokines

    • Claire Reynolds-Peterson, Dylan J. Ehrbar, Susanne M. McHale, Timothy J. LaRocca, Nicholas J. Mantis
    Pages 19-42

  4. Inhibition of Chondroitin Sulfate Proteoglycans by APRIL

    • Mashal Claude Ahmed, Bertrand Huard
    Pages 43-61

  5. Induction of Antigen-Independent Proliferation of Regulatory T-Cells by TNF Superfamily Ligands OX40L and GITRL

    • Prabhakaran Kumar, Zarema H. Arbieva, Mark Maienschein-Cline, Balaji B. Ganesh, Suresh Ramasamy, Bellur S. Prabhakar
    Pages 63-71

  6. Transition from TNF-Induced Inflammation to Death Signaling

    • Swati Choksi, Gourav Choudhary, Zheng-Gang Liu
    Pages 73-80

  7. Analysis of FcγR-Dependent Agonism of Antibodies Specific for Receptors of the Tumor Necrosis Factor (TNF) Receptor Superfamily (TNFRSF)

    • Juliane Medler, Harald Wajant
    Pages 81-90

  8. Generation and Evaluation of Bispecific Anti-TNF Antibodies Based on Single-Chain VHH Domains

    • M. A. Nosenko, K. -S. N. Atretkhany, V. V. Mokhonov, S. A. Chuvpilo, D. V. Yanvarev, M. S. Drutskaya et al.
    Pages 91-107

  9. In Vitro Physical and Functional Interaction Assays to Examine the Binding of Progranulin Derivative Atsttrin to TNFR2 and Its Anti-TNFα Activity

    • Wenyu Fu, Aubryanna Hettinghouse, Chuan-Ju Liu
    Pages 109-119

  10. Fluorescence-Based TNFR1 Biosensor for Monitoring Receptor Structural and Conformational Dynamics and Discovery of Small Molecule Modulators

    • Chih Hung Lo, Tory M. Schaaf, David D. Thomas, Jonathan N. Sachs
    Pages 121-137

  11. Production of Multi-Subtype Influenza Virus-Like Particles by Molecular Fusion with BAFF or APRIL for Vaccine Development

    • Ting-Hsuan Chen, Jo-Yu Hong, Chia-Chyi Liu, Chung-Chu Chen, Jia-Tsrong Jan, Suh-Chin Wu
    Pages 139-153

  12. Experimental Methods for the Immunological Characterization of Paradoxical Psoriasis Reactions Induced by TNF-α Biologics

    • Martina Morelli, Claudia Scarponi, Stefania Madonna, Cristina Albanesi
    Pages 155-165

  13. Methods for the Administration of EDAR Pathway Modulators in Mice

    • Sonia Schuepbach-Mallepell, Christine Kowalczyk-Quintas, Angela Dick, Mahya Eslami, Michele Vigolo, Denis J. Headon et al.
    Pages 167-183

  14. Analysis of Ligand-Receptor Interactions Using Bioluminescent TNF Superfamily (TNFSF) Ligand Fusion Proteins

    • Kirstin Kucka, Juliane Medler, Harald Wajant
    Pages 185-200

  15. Monitoring Atsttrin-Mediated Inhibition of TNFα/NF-κβ Activation Through In Vivo Bioluminescence Imaging

    • Aubryanna Hettinghouse, Wenyu Fu, Chuan-Ju Liu
    Pages 201-210

  16. The Use of Murine Infection Models to Investigate the Protective Role of TNF in Central Nervous System Tuberculosis

    • Nai-Jen Hsu, Muazzam Jacobs
    Pages 211-220

  17. Identification of CD137- and CD137L-Expressing Cells in EL-4 Tumor

    • Sang W. Kang, Hong R. Cho, Byungsuk Kwon
    Pages 221-229

  18. Expression Profiling of Tumor Necrosis Factor Superfamily Ligands mRNA in Healthy and Injured Murine Kidneys

    • Bhawna Tomar, Shrikant R. Mulay
    Pages 231-241

  19. Analysis of Lymphotoxin Alpha Expression in Human Retina and Generation of Expression Vectors to Functional Characterization of Polymorphisms in the Tumor Necrosis Factor Locus

    • Ricardo Usategui-Martín, Irene Rodriguez-Hernández, Rogelio González-Sarmiento, Eva M. Sobas, Jose Carlos Pastor-Jimeno, Salvador Pastor-Idoate
    Pages 243-250

  20. Detection of TNF-α Protein in Extracellular Vesicles Derived from Tumor Cells by Western Blotting

    • Tandressa Souza Berguetti, Raquel Ciuvalschi Maia, Paloma Silva de Souza
    Pages 251-258
Back to top

Keywords

About this book

This second edition focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Chapters detail protocols on the signaling process of TNF family members, technical examples to investigating the role of TNF family members in physiopathologies, modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, methodologies for various assays of TNF family members and the production of recombinant molecules.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, The TNF Superfamily: Methods and Applications, Second Edition serves to aid researchers investigating this key family of proteins.

Editors and Affiliations

  • INSERM, Sorbonne Université, Paris, France

    Jagadeesh Bayry

Bibliographic Information

  • Book Title: The TNF Superfamily

  • Book Subtitle: Methods and Protocols

  • Editors: Jagadeesh Bayry

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1130-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1129-6Published: 14 November 2020

  • Softcover ISBN: 978-1-0716-1132-6Published: 14 November 2021

  • eBook ISBN: 978-1-0716-1130-2Published: 13 November 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 284

  • Number of Illustrations: 14 b/w illustrations, 42 illustrations in colour

  • Topics: Protein Science

Publish with us

Policies and ethics

Back to top

Search

Navigation