Methods in Molecular Biology

Chondrocytes

Methods and Protocols

Editors: Haqqi, Tariq M., Lefebvre, Véronique (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $149.00
price for USA in USD
  • ISBN 978-1-0716-1119-7
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Hardcover $199.99
price for USA in USD
  • ISBN 978-1-0716-1118-0
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  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions & severe weather in the US may cause delays
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About this book

This detailed book collects a variety of methods from investigators at the forefront of chondrocyte biology and pathology research that they have tailored for their research projects in order to tackle the unique properties and challenges of the chondrocyte and those of its tissue matrix. Divided into three parts, the volume explores experimental models to study chondrocytes, in vivo assays, as well as methods to characterize the chondrocyte phenotype with high-throughput or discrete assays on cells maintained in vivo, just isolated from their tissue (ex vivo), or cultured in vitro. Written in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and cutting-edge, Chondrocytes: Methods and Protocols is an ideal guide for experts as well as newcomers in the cartilage field with the aim of sparking new research ideas in order to solve many of the pending mysteries regarding cartilage biology, disease mechanisms, and potential treatments.

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Isolation of Chondrocytes from Human Cartilage and Cultures in Monolayer and 3D

    Pages 1-12

    Somoza, Rodrigo A. (et al.)

  • Protocol for the Isolation of Intact Chondrons from Healthy and Osteoarthritic Human Articular Cartilage

    Pages 13-22

    Uzieliene, Ilona (et al.)

  • Cartilage-Specific Cre Recombinase Transgenes/Alleles in the Mouse

    Pages 23-38

    Kanakis, Ioannis (et al.)

  • Isolation of Mouse Growth Plate and Articular Chondrocytes for Primary Cultures

    Pages 39-51

    Haseeb, Abdul (et al.)

  • Fetal Growth Plate Cartilage : Histological and Immunohistochemical Techniques

    Pages 53-84

    Tata, Zachary (et al.)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-0716-1119-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-0716-1118-0
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions & severe weather in the US may cause delays
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Chondrocytes
Book Subtitle
Methods and Protocols
Editors
  • Tariq M. Haqqi
  • Véronique Lefebvre
Series Title
Methods in Molecular Biology
Series Volume
2245
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-1119-7
DOI
10.1007/978-1-0716-1119-7
Hardcover ISBN
978-1-0716-1118-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 229
Number of Illustrations
5 b/w illustrations, 41 illustrations in colour
Topics