Chondrocytes
Methods and Protocols
Editors: Haqqi, Tariq M., Lefebvre, Véronique (Eds.)
Free Preview- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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- About this book
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This detailed book collects a variety of methods from investigators at the forefront of chondrocyte biology and pathology research that they have tailored for their research projects in order to tackle the unique properties and challenges of the chondrocyte and those of its tissue matrix. Divided into three parts, the volume explores experimental models to study chondrocytes, in vivo assays, as well as methods to characterize the chondrocyte phenotype with high-throughput or discrete assays on cells maintained in vivo, just isolated from their tissue (ex vivo), or cultured in vitro. Written in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Chondrocytes: Methods and Protocols is an ideal guide for experts as well as newcomers in the cartilage field with the aim of sparking new research ideas in order to solve many of the pending mysteries regarding cartilage biology, disease mechanisms, and potential treatments.
- Table of contents (15 chapters)
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Isolation of Chondrocytes from Human Cartilage and Cultures in Monolayer and 3D
Pages 1-12
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Protocol for the Isolation of Intact Chondrons from Healthy and Osteoarthritic Human Articular Cartilage
Pages 13-22
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Cartilage-Specific Cre Recombinase Transgenes/Alleles in the Mouse
Pages 23-38
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Isolation of Mouse Growth Plate and Articular Chondrocytes for Primary Cultures
Pages 39-51
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Fetal Growth Plate Cartilage : Histological and Immunohistochemical Techniques
Pages 53-84
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Table of contents (15 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Chondrocytes
- Book Subtitle
- Methods and Protocols
- Editors
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- Tariq M. Haqqi
- Véronique Lefebvre
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2245
- Copyright
- 2021
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-1119-7
- DOI
- 10.1007/978-1-0716-1119-7
- Hardcover ISBN
- 978-1-0716-1118-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIII, 229
- Number of Illustrations
- 5 b/w illustrations, 41 illustrations in colour
- Topics
