- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Buy this book
- About this book
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This second edition of Eosinophils: Methods and Protocols updates several techniques from the first edition together with novel in vitro and in vivo-based methodologies. Written by internationally recognized expert authorities, this volume provides vital techniques from eosinophil purification to experimental modelling, with each technique spelled out in clear and straightforward terms, assuming no previous knowledge of the method and without necessitating sourcing additional information elsewhere. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, with tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Eosinophils: Methods and Protocols, Second Edition provides the practical means to extend our knowledge of eosinophil function in health and disease, underpinning research that may in turn lead to new hypotheses for future examinations into the role of this intriguing and enigmatic leukocyte.
- Table of contents (21 chapters)
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Molecular Biology of Eosinophils: Introduction
Pages 1-14
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Functionally Active Eosinophil Purification from Peripheral Blood
Pages 15-25
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Eosinophil Purification from Peripheral Blood of Rhesus Monkeys
Pages 27-35
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Generation of Mouse Eosinophils in Tissue Culture from Unselected Bone Marrow Progenitors
Pages 37-47
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Detection of Mouse Eosinophils in Tissue by Flow Cytometry and Isolation by Fluorescence-Activated Cell Sorting (FACS)
Pages 49-58
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Table of contents (21 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Eosinophils
- Book Subtitle
- Methods and Protocols
- Editors
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- Garry M. Walsh
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2241
- Copyright
- 2021
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-1095-4
- DOI
- 10.1007/978-1-0716-1095-4
- Hardcover ISBN
- 978-1-0716-1094-7
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XII, 297
- Number of Illustrations
- 14 b/w illustrations, 51 illustrations in colour
- Topics
