Skip to main content
SpringerLink
Log in

Toxicity Assessment

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Carlos Manuel Marques Palmeira

    1. MitoLab, Department of Life Sciences, Center for Neurosciences and Cell Biology of the University of Coimbra, Coimbra, Portugal

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Danielle Palma de Oliveira

    1. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Daniel Junqueira Dorta

    1. Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail reproducible for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2240)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. The Single-Cell Gel Electrophoresis Genotoxin Sensitivity Assay

    • Franziska Ferk, Miroslav Mišík, Halh Al-Serori, Armen Nersesyan, Georg Wultsch, Siegfried Knasmueller
    Pages 1-12

  3. Skin Sensitization Tests: The LLNA and the RhE IL-18 Potency Assay

    • Emanuela Corsini, Sue Gibbs, Erwin Roggen, Ian Kimber, David A. Basketter
    Pages 13-29

  4. Skin Equivalent Models: Protocols for In Vitro Reconstruction for Dermal Toxicity Evaluation

    • Tatiana do Nascimento Pedrosa, Carolina Motter Catarino, Paula Comune Pennacchi, Silvia Berlanga de Moraes Barros, Silvya Stuchi Maria-Engler
    Pages 31-41

  5. Intravital Microscopy: A Tool to Investigate the Toxicity in the Immune System , Vessel Rheology , and Xenobiotic Distribution

    • Cristina Bichels Hebeda, Éric Diego Barioni, Sandra Helena Poliselli Farsky
    Pages 43-55

  6. Cell-Based Biosensor to Visualize Nitric Oxide Release from Living Cells for Toxicity Assessment

    • Moritoshi Sato, Yoshio Umezawa
    Pages 57-64

  7. In Vitro Contraction of Isolated Cauda Epididymal Duct Smooth Muscle as a Complimentary Approach to Physiological, Pathological, Toxicological, and Pharmacological Studies on Epididymal Function

    • Cibele S. Borges, Luiz Ricardo A. Kiguti, André S. Pupo, Wilma G. Kempinas
    Pages 65-76

  8. Early Life Stage Assays in Zebrafish

    • Flávia Renata Abe, Klaus Alvaro Guerrieri Accoroni, Carlos Gravato, Danielle Palma de Oliveira
    Pages 77-92

  9. Assessment of Urothelial Cytotoxicity at Morphological and Molecular Levels: Urinary Bladder as Target Organ

    • Thania Rios Rossi Lima, Bianca Camargo Penteado Sales, Shadia Muhammad Ihlaseh Catalano
    Pages 93-102

  10. Evaluation of Development of the Rat Uterus as a Toxicity Biomarker

    • Marlise Guerrero Schimpf, María M. Milesi, Enrique H. Luque, Jorgelina Varayoud
    Pages 103-117

  11. Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish

    • Carlos Gravato, Flávia Renata Abe, Danielle Palma de Oliveira, Amadeu M. V. M. Soares, Inês Domingues
    Pages 119-124

  12. Ecotoxicity Assays Using Freshwater Planarians

    • João Luís Teixeira Pestana, Pearl Ugochi Ofoegbu
    Pages 125-137

  13. Toxicogenomic Analysis

    • Taisa Magnani Dinamarco, Paula Fagundes de Gouvêa, Emerson de Souza Santos, Lucas Oliveira Sousa
    Pages 139-174

  14. Strategy Combining Nonanimal Methods for Ocular Toxicity Evaluation

    • Marize Campos Valadares, Gisele Augusto Rodrigues de Oliveira, Renato Ivan de Ávila, Artur Christian Garcia da Silva
    Pages 175-195

  15. Immunocytochemistry Analysis of HepG2 Cell 3D Culture Encapsulated as Spheroids in Alginate Beads

    • Raul Ghiraldelli Miranda, Elisa Raquel Anastácio Ferraz, Lilian Cristina Pereira, Daniel Junqueira Dorta
    Pages 197-206

  16. Oxygen Consumption Evaluation: An Important Indicator of Metabolic State, Cellular Function , and Cell Fate Along Neural Deregulation

    • Mariana Dutra Brito, Luiz Felipe Souza e Silva, Amanda Siena, Miruna Chipara, Sovan Sarkar, Tatiana Rosado Rosenstock
    Pages 207-230

  17. Mitochondrial Bioenergetic Assays as a Standard Protocol for Toxicological and Metabolic Assessment

    • João Soeiro Teodoro, Anabela Pinto Rolo, Carlos Manuel Marques Palmeira
    Pages 231-241

  18. In Vitro Apoptotic Cell Death Assessment

    • Lilian Cristina Pereira, Alecsandra Oliveira de Souza, Raul Ghiraldelli Miranda, Daniel Junqueira Dorta
    Pages 243-261

  19. Back Matter

    Pages 263-266
    Download chapter PDF
Back to top

Keywords

About this book

This book collects protocols from different areas of knowledge to assist in the identification of toxic effects exerted by different xenobiotics. At the same time as classical techniques are presented, modern techniques with alternative models to the use of animals are also presented. Given the ever-increasing exposure to different compounds and their effects on population health, the assessment of multiple endpoints is of utmost importance for better risk assessment, and this collection addresses that need. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Toxicity Assessment: Methods and Protocols aims to serve researchers in this vast field of science as they seek to better understand the mechanisms of action of different xenobiotics.

Editors and Affiliations

  • MitoLab, Department of Life Sciences, Center for Neurosciences and Cell Biology of the University of Coimbra, Coimbra, Portugal

    Carlos Manuel Marques Palmeira

  • School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

    Danielle Palma de Oliveira

  • Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

    Daniel Junqueira Dorta

Bibliographic Information

  • Book Title: Toxicity Assessment

  • Book Subtitle: Methods and Protocols

  • Editors: Carlos Manuel Marques Palmeira, Danielle Palma de Oliveira, Daniel Junqueira Dorta

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1091-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1090-9Published: 10 January 2021

  • Softcover ISBN: 978-1-0716-1093-0Published: 10 January 2022

  • eBook ISBN: 978-1-0716-1091-6Published: 09 January 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 266

  • Number of Illustrations: 10 b/w illustrations, 68 illustrations in colour

  • Topics: Biomedicine, general

Publish with us

Policies and ethics

Back to top

Search

Navigation