Rice Genome Engineering and Gene Editing
Methods and Protocols
Editors: Bandyopadhyay, Anindya, Thilmony, Roger (Eds.)
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- Provides step-by-step detail essential for reproducible results
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- About this book
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This detailed volume explores rice molecular biology, genetic engineering, and genome editing technologies. Dividing into three parts, the book covers subjects such as genetic engineering and tissue culture of rice, including efficient methods for rice transformation and regeneration, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR systems for targeted gene knock-out or genome modification via base editing, and diverse methods describing bioinformatic, molecular, and cellular analyses in rice. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Rice Genome Engineering and Gene Editing: Methods and Protocols serves as a valuable resource for researchers worldwide striving to further their efforts on advancing research and producing genetically improved rice varieties.
- Table of contents (23 chapters)
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Gene Assembly in Agrobacterium via Nucleic Acid Transfer Using Recombinase Technology (GAANTRY)
Pages 3-17
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TransGene Stacking II Vector System for Plant Metabolic Engineering and Synthetic Biology
Pages 19-35
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Morphogenic Regulators and Their Application in Improving Plant Transformation
Pages 37-61
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A Rapid Method for Stably Transforming Rice Seeds
Pages 63-68
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Agrobacterium tumefaciens-Mediated Transformation of Rice by Hygromycin Phosphotransferase (hptII) Gene Containing CRISPR/Cas9 Vector
Pages 69-79
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Table of contents (23 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Rice Genome Engineering and Gene Editing
- Book Subtitle
- Methods and Protocols
- Editors
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- Anindya Bandyopadhyay
- Roger Thilmony
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2238
- Copyright
- 2021
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-1068-8
- DOI
- 10.1007/978-1-0716-1068-8
- Hardcover ISBN
- 978-1-0716-1067-1
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XV, 378
- Number of Illustrations
- 11 b/w illustrations, 70 illustrations in colour
- Topics
