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  • © 2021

Myeloid-Derived Suppressor Cells

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2236)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xii
  2. Isolation and Phenotyping of Splenic Myeloid-Derived Suppressor Cells in Murine Cancer Models

    • Emilio Sanseviero, Rina Kim, Dmitry I. Gabrilovich
    Pages 19-28
  3. Phenotypical Characterization and Isolation of Tumor-Derived Mouse Myeloid-Derived Suppressor Cells

    • Roza Maria Barouni, Chiara Musiu, Vincenzo Bronte, Stefano Ugel, Stefania Canè
    Pages 29-42
  4. High-Dimensional Phenotyping of Human Myeloid-Derived Suppressor Cells/Tumor-Associated Macrophages in Tissue by Mass Cytometry

    • Juliette Ferrant, Simon Le Gallou, Guillaume Manson, Steve Genebrier, Frederic Mourcin, Karin Tarte et al.
    Pages 57-66
  5. Depletion and Maturation of Myeloid-Derived Suppressor Cells in Murine Cancer Models

    • Christopher Groth, Rebekka Weber, Jochen Utikal, Viktor Umansky
    Pages 67-75
  6. Measuring Suppressive Activity and Autophagy in Myeloid-Derived Suppressor Cells

    • Antonis Stylianos Papaioannou, Athina Boumpas, Miranta Papadopoulou, Aikaterini Hatzioannou, Themis Alissafi, Panayotis Verginis
    Pages 85-98
  7. High-Dimensional Analysis of Circulating and Tissue-Derived Myeloid-Derived Suppressor Cells from Patients with Glioblastoma

    • Tyler J. Alban, Defne Bayik, Alvaro G. Alvarado, Harley I. Kornblum, Justin D. Lathia
    Pages 157-175
  8. Single-Cell Transcriptome Analysis Workflow for Splenic Myeloid-Derived Suppressor Cells from Murine Breast Cancer Models

    • Hamad Alshetaiwi, Nicholas Pervolarakis, Quy H. Nguyen, Kai Kessenbrock
    Pages 177-187
  9. Intravital 2-Photon Microscopy of Diverse Cell Types in the Murine Tibia

    • Anja Hasenberg, Lucas Otto, Matthias Gunzer
    Pages 189-201
  10. Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting

    • Marion Rambault, Rachana Borkute, Emilie Doz-Deblauwe, Yves Le-Vern, Nathalie Winter, Anca Dorhoi et al.
    Pages 203-217
  11. Back Matter

    Pages 219-220

About this book

This volume explores various experimental approaches that can be used to investigate myeloid-derived suppressor cells (MDSC) and can be useful to understand the characterization of these cells. The chapters in this book cover topics such as the study of MDSC in human patient samples; employing flow cytometry or magnetic enrichment; immunophenotyping using multi-parameter flow cytometry and mass cytometry; functional characterization of MDSC by biochemical, immunological, and microscopy approaches; MDSC characterization through the use of high-resolution genomics; and novel methods of high-resolution visualization of living tissue and the purification of granulocytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and thorough, Myeloid-Derived Suppressor Cells: Methods and Protocols is a valuable resource for novices in this field to help them start their research on MDSC, and experienced investigators looking to establish new protocols in their lab. This book is great for anyone working in the field of biology, medicine, and veterinary medicine.  


Editors and Affiliations

  • Experimental and Translational Research, Department of Otorhinolaryngology, University Hospital Essen, West German Cancer Center, Essen, Germany

    Sven Brandau

  • Institute of Immunology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany

    Anca Dorhoi

Bibliographic Information

  • Book Title: Myeloid-Derived Suppressor Cells

  • Editors: Sven Brandau, Anca Dorhoi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1060-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1059-6Published: 26 November 2020

  • Softcover ISBN: 978-1-0716-1062-6Published: 27 November 2021

  • eBook ISBN: 978-1-0716-1060-2Published: 25 November 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 220

  • Number of Illustrations: 21 b/w illustrations, 36 illustrations in colour

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access