- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume explores the techniques used to study human and animal pericytes. Chapters in this volume cover a range of topics such as pericyte dissociation from tissues, and analysis by immunohistochemistry and flow cytometry; avian chimaeras to investigate the embryonic development of cell lineage; pericyte purification by fluorescence activated cell sorting and ensuing culture; tissue turnover and regeneration; and the dynamic study of pericyte involvement in cancer dissemination, in situ, and in culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Pericytes: Methods and Protocols is a valuable resource that helps researchers define the biologic identity, functions, and potentials of a cell lineage of increasing interest and therapeutic significance.
- Table of contents (13 chapters)
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Visualizing Pericyte Mimicry of Angiotropic Melanoma by Direct Labeling of the Angioarchitecture
Pages 1-12
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Transplantation of Purified Human Pericytes for Myocardial Repair in SCID-hu Mice
Pages 13-26
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Assessment of Pericyte Phenotype by Flow Cytometry
Pages 27-35
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Characterization of Human Pericyte Phenotype by Immunohistochemistry
Pages 37-45
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A Metabolomics-Based Approach to Identify Lineage Guiding Molecules in Pericyte Cultures
Pages 47-59
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Table of contents (13 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Pericytes
- Book Subtitle
- Methods and Protocols
- Editors
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- Bruno Péault
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2235
- Copyright
- 2021
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-1056-5
- DOI
- 10.1007/978-1-0716-1056-5
- Hardcover ISBN
- 978-1-0716-1055-8
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 192
- Number of Illustrations
- 3 b/w illustrations, 26 illustrations in colour
- Topics
