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Trichoderma reesei

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Astrid R. Mach-Aigner

    1. Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

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  2. Roland Martzy

    1. Christian Doppler Laboratory for Optimized Expression of Carbohydrate-Active Enzymes, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2234)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
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  2. Ecological Genomics and Evolution of Trichoderma reesei

    • Komal Chenthamara, Irina S. Druzhinina, Mohammad J. Rahimi, Marica Grujic, Feng Cai
    Pages 1-21

  3. Industrial Relevance of Trichoderma reesei as an Enzyme Producer

    • Amanda J. Fischer, Suchindra Maiyuran, Debbie S. Yaver
    Pages 23-43

  4. The Potential of Synthetic Biology for Trichoderma reesei

    • Roland Martzy, Astrid R. Mach-Aigner
    Pages 45-54

  5. Resistance Marker- and Gene Gun-Mediated Transformation of Trichoderma reesei

    • Monika Schmoll, Susanne Zeilinger
    Pages 55-62

  6. Use of Auxotrophic Markers for Targeted Gene Insertions in Trichoderma reesei

    • Irene Tomico-Cuenca, Christian Derntl
    Pages 63-72

  7. Open the Pores: Electroporation for the Transformation of Trichoderma reesei

    • Franziska Wanka
    Pages 73-78

  8. Sexual Crossing of Trichoderma reesei

    • Rita B. Linke
    Pages 79-85

  9. CRISPR/Cas9-Mediated Genome Editing of Trichoderma reesei

    • Gen Zou, Zhihua Zhou
    Pages 87-98

  10. The Copper-Controlled RNA Interference System in Trichoderma reesei

    • Lei Wang, Weixin Zhang, Xiangfeng Meng, Weifeng Liu
    Pages 99-111

  11. Batch Cultivation of Trichoderma reesei

    • Birgit Jovanović
    Pages 113-118

  12. Image Analysis Method for the Characterization of Trichoderma reesei During Fermentations

    • Nicolas Hardy, Maxime Moreaud, Fadhel Ben Chaabane
    Pages 119-133

  13. Measurement of Cellulase and Xylanase Activities in Trichoderma reesei

    • Qing-Shan Meng, Fei Zhang, Chen-Guang Liu, Feng-Wu Bai, Xin-Qing Zhao
    Pages 135-146

  14. Flow Cytometry for Filamentous Fungi

    • Matthias G. Steiger
    Pages 147-155

  15. Molecular Identification of Trichoderma reesei

    • Mohammad J. Rahimi, Feng Cai, Marica Grujic, Komal Chenthamara, Irina S. Druzhinina
    Pages 157-175

  16. In Vivo Footprinting Analysis in Trichoderma reesei

    • Alice Rassinger
    Pages 177-189

  17. RNA Characterization in Trichoderma reesei

    • Petra Till
    Pages 191-235

  18. Proteomic Profiling of the Secretome of Trichoderma reesei

    • So Fong Cam Ngan, Siu Kwan Sze
    Pages 237-249

  19. Transcriptomics in Trichoderma reesei

    • Amanda C. C. Antonieto, Roberto N. Silva
    Pages 251-269

  20. The Comprehensive and Reliable Detection of Secondary Metabolites in Trichoderma reesei: A Tool for the Discovery of Novel Substances

    • Bernhard Seidl, Christoph Bueschl, Rainer Schuhmacher
    Pages 271-295
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Keywords

About this book

This detailed book collects standard techniques and cutting-edge methods that are frequently used by the research community studying the fungus Trichoderma reesei. Beginning with overviews of its evolution, its use in the production of industrially-relevant enzymes, and synthetic biology applications, the volume continues with methods covering topics from transformation techniques and gene editing to downstream-analytical applications and -omics analyses and the corresponding bioinformatics approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Trichoderma reesei: Methods and Protocols serves as an ideal guide for a wide range of audiences, from students who want to familiarize themselves with basic research protocols to experienced scientists who are planning to establish a new method in their laboratories, working with this dynamic fungus.

Editors and Affiliations

  • Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

    Astrid R. Mach-Aigner

  • Christian Doppler Laboratory for Optimized Expression of Carbohydrate-Active Enzymes, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

    Roland Martzy

Bibliographic Information

  • Book Title: Trichoderma reesei

  • Book Subtitle: Methods and Protocols

  • Editors: Astrid R. Mach-Aigner, Roland Martzy

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1048-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1047-3Published: 10 November 2020

  • Softcover ISBN: 978-1-0716-1050-3Published: 10 November 2021

  • eBook ISBN: 978-1-0716-1048-0Published: 09 November 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 364

  • Number of Illustrations: 17 b/w illustrations, 46 illustrations in colour

  • Topics: Mycology, Microbiology, Enzymology

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