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Methods in Molecular Biology

Exocytosis and Endocytosis

Methods and Protocols

Editors: Niedergang, Florence, Vitale, Nicolas, Gasman, Stéphane (Eds.)

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  • Includes cutting-edge methods and protocols
    Provides step-by-step detail essential for reproducible results
    Contains key notes and implementation advice from the experts

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eBook $149.00
price for USA in USD
  • ISBN 978-1-0716-1044-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
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About this book

This volume provides basic and cutting-edge methods and protocols to study the major characteristics of eukaryotic cells.  Chapters detail the different pathways of endocytosis in vivo, real time imaging of endocytic steps, endocytosis in model organisms, super-resolution methods to follow proteins involved in exocytosis, specific protocols for exocytosis in specialized cells such as neutrophils or neuroendocrine cells, as well as secretion of exosomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and clearly written, Exocytosis and Endocytosis: Methods and Protocols is a valuable resource for researchers in the fields of cell biology, neurology, immunology, oncology, and those interested in studying protein trafficking and signal regulation.


Table of contents (21 chapters)

Table of contents (21 chapters)
  • Stoichiometry of Receptors at the Plasma Membrane During Their Endocytosis Using Total Internal Reflection Fluorescent (TIRF) Microscopy Live Imaging and Single-Molecule Tracking

    Pages 3-17

    Salavessa, Laura (et al.)

  • Measuring Endocytosis During Proliferative Cell Quiescence

    Pages 19-42

    Hinze, Claudia (et al.)

  • Measurements of Compensatory Endocytosis by Antibody Internalization and Quantification of Endocytic Vesicle Distribution in Adrenal Chromaffin Cells

    Pages 43-51

    Ceridono, Mara (et al.)

  • Quantitative Methods to Study Endocytosis and Retrograde Transport of Cargo Proteins

    Pages 53-70

    Shafaq-Zadah, Massiullah (et al.)

  • High-Content Drug Discovery Screening of Endocytosis Pathways

    Pages 71-91

    Cardoso, David A. (et al.)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-0716-1044-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Exocytosis and Endocytosis
Book Subtitle
Methods and Protocols
Editors
  • Florence Niedergang
  • Nicolas Vitale
  • Stéphane Gasman
Series Title
Methods in Molecular Biology
Series Volume
2233
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-1044-2
DOI
10.1007/978-1-0716-1044-2
Hardcover ISBN
978-1-0716-1043-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 329
Number of Illustrations
12 b/w illustrations, 72 illustrations in colour
Topics