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Skeletal Development and Repair

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Matthew J. Hilton

    1. Department of Orthopaedic Surgery and Cell Biology, Duke Cellular, Developmental, and Genome Laboratories, Duke University School of Medicine, Durham, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2230)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
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  2. Murine Skeletal Development, Repair, and Genetic Models

    1. Front Matter

      Pages 1-1
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    2. Overview of Skeletal Development

      • Tatsuya Kobayashi, Henry M. Kronenberg
      Pages 3-16

    3. Overview of Skeletal Repair (Fracture Healing and Its Assessment)

      • Elise F. Morgan, Anthony De Giacomo, Louis C. Gerstenfeld
      Pages 17-37

    4. Advantages and Limitations of Cre Mouse Lines Used in Skeletal Research

      • Florent Elefteriou, Greig Couasnay
      Pages 39-59

  3. Skeletal Repair, Parabiosis, Transplantations, and Organ Cultures

    1. Front Matter

      Pages 61-61
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    2. Generation of Closed Transverse Fractures in Small Animals

      • Anthony De Giacomo, Elise F. Morgan, Louis C. Gerstenfeld
      Pages 63-73

    3. A Mouse Femoral Ostectomy Model to Assess Bone Graft Substitutes

      • Ryan P. Trombetta, Emma K. Knapp, Hani A. Awad
      Pages 75-89

    4. Surgical Induction of Posttraumatic Osteoarthritis in the Mouse

      • Robert D. Maynard, David A. Villani, William G. Schroeder, Douglas J. Adams, Michael J. Zuscik
      Pages 91-103

    5. Parabiosis: Assessing the Effects of Circulating Cells and Factors on the Skeleton

      • Benjamin Alman, Gurpreet Baht
      Pages 105-113

    6. Murine Limb Bud Organ Cultures for Studying Musculoskeletal Development

      • Martin Arostegui, T. Michael Underhill
      Pages 115-137

    7. Murine Limb Explant Cultures to Assess Cartilage Development

      • Manuela Wuelling, Andrea Vortkamp
      Pages 139-149

    8. Renal Capsule Transplantation to Assay Angiogenesis in Skeletal Development and Repair

      • Anais Julien, Simon Perrin, Rana Abou-Khalil, Céline Colnot
      Pages 151-165

  4. Structural, Histological, and Molecular Analyses on Skeletal Tissues and Tissue Sections

    1. Front Matter

      Pages 167-167
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    2. MicroCT for Scanning and Analysis of Mouse Bones

      • Yung Kim, Michael D. Brodt, Simon Y. Tang, Matthew J. Silva
      Pages 169-198

    3. Four-Point Bending Testing for Mechanical Assessment of Mouse Bone Structural Properties

      • Hattie C. Cutcliffe, Louis E. DeFrate
      Pages 199-215

    4. Phenotyping Intact Mouse Bones Using Bone CLARITY

      • Jennifer B. Treweek, Aidan Beres, Nathan Johnson, Alon Greenbaum
      Pages 217-230

    5. Processing and Sectioning Undecalcified Murine Bone Specimens

      • Thomas B. Bemenderfer, Jonathan S. Harris, Keith W. Condon, Jiliang Li, Melissa A. Kacena
      Pages 231-257

    6. Preparation of Thin Frozen Sections from Nonfixed and Undecalcified Hard Tissues Using Kawamoto’s Film Method (2020)

      • Tadafumi Kawamoto, Komei Kawamoto
      Pages 259-281

    7. Demineralized Murine Skeletal Histology

      • Anthony J. Mirando, Matthew J. Hilton
      Pages 283-302
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Keywords

About this book

This second edition provides a comprehensive laboratory manual on skeletal development and skeletal repair research utilizing mouse models. Chapters provide methods and protocols on the most current and cutting-edge techniques in skeletal development and repair, histological, cellular, and molecular techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Skeletal Development and Repair: Methods and Protocols-Second Edition aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Orthopaedic Surgery and Cell Biology, Duke Cellular, Developmental, and Genome Laboratories, Duke University School of Medicine, Durham, USA

    Matthew J. Hilton

Bibliographic Information

  • Book Title: Skeletal Development and Repair

  • Book Subtitle: Methods and Protocols

  • Editors: Matthew J. Hilton

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1028-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1027-5Published: 17 November 2020

  • Softcover ISBN: 978-1-0716-1030-5Published: 17 November 2021

  • eBook ISBN: 978-1-0716-1028-2Published: 16 November 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 482

  • Number of Illustrations: 20 b/w illustrations, 105 illustrations in colour

  • Topics: Biomedicine, general, Regenerative Medicine/Tissue Engineering

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