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Developmental Biology of the Sea Urchin and Other Marine Invertebrates

Methods and Protocols

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2219)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiii
  2. The Starfish Asterina pectinifera: Collection and Maintenance of Adults and Rearing and Metamorphosis of Larvae

    • Naoyuki Murabe, Ei-ichi Okumura, Kazuyoshi Chiba, Enako Hosoda, Susumu Ikegami, Takeo Kishimoto
    Pages 49-68
  3. Staining and Tracking Methods for Studying Sponge Cell Dynamics

    • Carole Borchiellini, Sandie M. Degnan, Emilie Le Goff, Caroline Rocher, Amélie Vernale, Stephen Baghdiguian et al.
    Pages 81-97
  4. Microscopy Studies of Placozoans

    • Carolyn L. Smith, Tatiana D. Mayorova, Christine A. Winters, Thomas S. Reese, Sally P. Leys, Andreas Heyland
    Pages 99-118
  5. Recovery of Sea Star Egg Cell Surface Proteins Released at Fertilization

    • Emily Wiseman, Lauren Bates, David J. Carroll
    Pages 151-161
  6. Quantifying Cell Proliferation During Regeneration of Aquatic Worms

    • Eduardo E. Zattara, B. Duygu Özpolat
    Pages 163-180
  7. In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis

    • Laura Fierro-Constaín, Caroline Rocher, Florent Marschal, Quentin Schenkelaars, Nina Séjourné, Carole Borchiellini et al.
    Pages 181-194
  8. Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond

    • Margherita Perillo, Periklis Paganos, Maxwell Spurrell, Maria I. Arnone, Gary M. Wessel
    Pages 195-216
  9. Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation

    • Alex McDougall, Celine Hebras, Isa Gomes, Remi Dumollard
    Pages 217-230
  10. Transcriptomic Analysis in the Sea Anemone Nematostella vectensis

    • Jacob F. Warner, Eric Röttinger
    Pages 231-240
  11. RNA Interference on Regenerating Holothurian Gut Tissues

    • Miosotis Alicea-Delgado, Samir A. Bello-Melo, José E. García-Arrarás
    Pages 241-252
  12. ATAC-Seq for Assaying Chromatin Accessibility Protocol Using Echinoderm Embryos

    • Marta S. Magri, Danila Voronov, Jovana RanÄ‘elović, Claudia Cuomo, Jose Luis Gómez-Skarmeta, Maria I. Arnone
    Pages 253-265
  13. Usage of the Sea Urchin Hemicentrotus pulcherrimus Database, HpBase

    • Sonoko Kinjo, Masato Kiyomoto, Takashi Yamamoto, Kazuho Ikeo, Shunsuke Yaguchi
    Pages 267-275
  14. Functional Studies of Trichoplax adhaerens Voltage-Gated Calcium Channel Activity

    • Julia Gauberg, Adriano Senatore, Andreas Heyland
    Pages 277-288
  15. A Bioinformatics Tutorial for Comparative Development Genomics in Diverse Meiofauna

    • Joseph L. Sevigny, Jon L. Norenburg, Francesca Leasi
    Pages 289-305

About this book

This detailed second edition presents a wide variety of marine invertebrate model systems, from cephalochordata to holothurians, along with novel experimental protocols for taking advantage of their unique properties. The techniques range from culturing the organisms to modifying their DNA. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, Second Edition is an ideal guide for researchers working with these versatile organisms and for furthering our understanding of fundamental biological questions.

Editors and Affiliations

  • Department of Biochemistry and Molecular Genetics, Midwestern University, Glendale, USA

    David J. Carroll

  • Department of Biology, University of New Mexico, Albuquerque, USA

    Stephen A. Stricker

Bibliographic Information

  • Book Title: Developmental Biology of the Sea Urchin and Other Marine Invertebrates

  • Book Subtitle: Methods and Protocols

  • Editors: David J. Carroll, Stephen A. Stricker

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0974-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0973-6Published: 20 October 2020

  • Softcover ISBN: 978-1-0716-0976-7Published: 21 October 2021

  • eBook ISBN: 978-1-0716-0974-3Published: 19 October 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 309

  • Number of Illustrations: 27 b/w illustrations, 48 illustrations in colour

  • Topics: Biological Techniques, Zoology, Developmental Biology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access