Methods in Molecular Biology

The Integrin Interactome

Methods and Protocols

Editors: Vicente-Manzanares, Miguel (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-0716-0962-0
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Hardcover $199.99
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  • ISBN 978-1-0716-0961-3
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About this book

This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.


Table of contents (17 chapters)

Table of contents (17 chapters)
  • Measurement of Integrin Activation and Conformational Changes on the Cell Surface by Soluble Ligand and Antibody Binding Assays

    Pages 3-15

    Wang, Zhengli (et al.)

  • Quantification of Integrin Activation and Ligation in Adherent Cells

    Pages 17-25

    Al-Yafeai, Zaki (et al.)

  • Multiparametric Analysis of Focal Adhesions in Bidimensional Substrates

    Pages 27-37

    Talayero, Vanessa C. (et al.)

  • Focal Adhesion Isolation Assay Using ECM-Coated Magnetic Beads

    Pages 39-44

    Sturgess, Wesley (et al.)

  • Functional Integrin Regulation Through Interactions with Tetraspanin CD9

    Pages 47-56

    Torres-Gómez, Álvaro (et al.)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-0716-0962-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-0716-0961-3
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions & severe weather in the US may cause delays
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
The Integrin Interactome
Book Subtitle
Methods and Protocols
Editors
  • Miguel Vicente-Manzanares
Series Title
Methods in Molecular Biology
Series Volume
2217
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0962-0
DOI
10.1007/978-1-0716-0962-0
Hardcover ISBN
978-1-0716-0961-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIX, 315
Number of Illustrations
11 b/w illustrations, 43 illustrations in colour
Topics