Methods in Molecular Biology

Epigenetic Reprogramming During Mouse Embryogenesis

Methods and Protocols

Editors: Ancelin, Katia, Borensztein, Maud (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-0716-0958-3
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About this book

This volume explores the latest techniques used to study and understand chromatin reprogramming in embryos and germ cells. Various culture systems are presented, which consist of invaluable tools to investigate many developmental processes. This book also looks at methods for direct examination of DNA, RNA, and proteins in embryos, along with low-input and single-cell assays for exploring these features at the genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Epigenetics Reprogramming During Mouse Embryogenesis: Methods and Protocols is a valuable resource for any scientist and researcher looking to make new discoveries in this fascinating field of chromatin reprogramming. 


Table of contents (20 chapters)

Table of contents (20 chapters)
  • Ex Vivo Culture for Preimplantation Mouse Embryo to Analyze Pluripotency

    Pages 1-10

    Boutourlinsky, Katia (et al.)

  • Microfabricated Device for High-Resolution Imaging of Preimplantation Embryos

    Pages 11-30

    Vandormael-Pournin, Sandrine (et al.)

  • In Vitro Culture of Mouse Blastocysts to the Egg Cylinder Stage via Mural Trophectoderm Excision

    Pages 31-40

    Ozguldez, Hatice O. (et al.)

  • Spatially Organized Differentiation of Mouse Pluripotent Stem Cells on Micropatterned Surfaces

    Pages 41-58

    Morgani, Sophie M. (et al.)

  • Mouse Primordial Germ Cells: In Vitro Culture and Conversion to Pluripotent Stem Cell Lines

    Pages 59-73

    Borkowska, Malgorzata (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-0716-0958-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-0716-0957-6
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Epigenetic Reprogramming During Mouse Embryogenesis
Book Subtitle
Methods and Protocols
Editors
  • Katia Ancelin
  • Maud Borensztein
Series Title
Methods in Molecular Biology
Series Volume
2214
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0958-3
DOI
10.1007/978-1-0716-0958-3
Hardcover ISBN
978-1-0716-0957-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 318
Number of Illustrations
16 b/w illustrations, 58 illustrations in colour
Topics