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Opioid Receptors

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Santi M. Spampinato

    1. Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2201)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Transcriptional and Post-Transcriptional Analysis and Cellular Detection of Opioid Receptors

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Overview of Genetic Analysis of Human Opioid Receptors

      • Santi M. Spampinato
      Pages 3-13

    3. Renilla Luciferase Reporter Assay to Study 3′-UTR-Driven Posttranscriptional Regulation of OPRM1

      • Gabriele Vincelli, Andrea Bedini
      Pages 15-26

    4. Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1

      • Vittoria Borgonetti, Nicoletta Galeotti
      Pages 27-34

    5. Monitoring Opioid Receptor Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET)

      • Monica Baiula
      Pages 35-43

    6. Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Nonvisual Arrestins

      • Andrea Bedini
      Pages 45-58

    7. Functionalization and Bioconjugation of Nanoruby for Long-Term, Ultrasensitive Imaging of Îœu-Opioid Receptors

      • Rashmi Pillai, Mark Connor, Varun K. A. Sreenivasan
      Pages 59-70

    8. Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues

      • Yvonne Schmidt, Halina Machelska
      Pages 71-82

    9. Real-Time Quantitative Reverse Transcription PCR for Detection of Opioid Receptors in Immune Cells

      • Melih Özgür Celik, Dominika Labuz, Halina Machelska
      Pages 83-95

  3. Analysis of Signaling Events Modulated by Opioid Receptors

    1. Front Matter

      Pages 97-97
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    2. Quantitative Analysis of MOR-1 Internalization in Spinal Cord of Morphine-Tolerant Mice

      • Vittoria Borgonetti, Nicoletta Galeotti
      Pages 99-108

    3. GTPγS-Autoradiography for Studies of Opioid Receptor Functionality

      • Alfhild Grönbladh, Mathias Hallberg
      Pages 109-116

    4. Evaluating Opioid-Mediated Adenylyl Cyclase Inhibition in Live Cells Using a BRET-Based Assay

      • Preeti Manandhar, Shivani Sachdev, Marina Santiago
      Pages 117-125

    5. Patch Clamp Analysis of Opioid-Induced Kir3 Currents in Mouse Peripheral Sensory Neurons Following Nerve Injury

      • Viola Seitz, Philip Stötzner, Dominika Labuz, Halina Machelska
      Pages 127-137

    6. Opioid Modulation of Neuronal Iron and Potential Contributions to NeuroHIV

      • Bradley Nash, Elena Irollo, Renato Brandimarti, Olimpia Meucci
      Pages 139-162

    7. An in Vitro Assay to Study the Role of Opioids in Modulating Immune Cell Adhesion

      • Monica Baiula
      Pages 163-169

    8. Assessing Cell Viability Effects of Opioids in Primary Cortical Cells from Rat

      • Alfhild Grönbladh, Erik Nylander, Sofia Zelleroth, Mathias Hallberg
      Pages 171-180

    9. The Quest for More Effective Analgesics with Reduced Abuse Liability and Fewer Adverse Effects: Promises, Pitfalls, and Future Perspectives of Biased Agonists at Opioid Receptors

      • Andrea Bedini
      Pages 181-192

  4. Model Systems to Studying Opioid Receptor-Mediated Functions

    1. Front Matter

      Pages 193-193
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Keywords

About this book

This second edition presents an up-to-date chapters describing the most relevant and novel techniques employed to study the opioid receptors. Chapters detail transcriptional and post-transcriptional analysis, cellular detection of opioid receptors, analysis of signaling events modulated by opioid receptors, model systems to studying opioid receptor-mediated functions, and behavioral effects mediated by opioid receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Opioid Receptors: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy

    Santi M. Spampinato

Bibliographic Information

  • Book Title: Opioid Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Santi M. Spampinato

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0884-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0883-8Published: 26 September 2020

  • Softcover ISBN: 978-1-0716-0886-9Published: 27 September 2021

  • eBook ISBN: 978-1-0716-0884-5Published: 25 September 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 270

  • Number of Illustrations: 18 b/w illustrations, 24 illustrations in colour

  • Topics: Receptors, Neurobiology

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