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DNA Modifications

Methods and Protocols

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2198)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xvi
  2. Introduction

    1. Front Matter

      Pages 1-1
    2. Mapping DNA Methylation in Mammals: The State of the Art

      • Antonio Lentini, Colm E. Nestor
      Pages 37-50
  3. Detection and Quantification of DNA Modifications Using Mass Spectrometry and Capillary Gel Electrophoresis

    1. Front Matter

      Pages 51-51
    2. Detection of Modified Bases in Bacteriophage Genomic DNA

      • Yan-Jiun Lee, Peter R. Weigele
      Pages 53-66
    3. Detection of DNA Methylation in Genomic DNA by UHPLC-MS/MS

      • Konstantinos Boulias, Eric Lieberman Greer
      Pages 79-90
    4. MS Analysis of DNA Modifications in Urinary/Body Fluids

      • Aleksandra Skalska, Agnieszka Siomek-Gorecka, Ryszard Olinski, Rafal Rozalski
      Pages 109-122
    5. Preparation of Internal Standards for 2D-UPLC-MS/MS Quantification of Noncanonical DNA Bases

      • Marta Starczak, Aleksandra Skalska, Rafal Rozalski, Ryszard Olinski, Daniel Gackowski
      Pages 123-136
    6. Detection of TET-Oxidized 5-Methylcytosine Bases by Capillary Gel Electrophoresis

      • Romualdas Vaisvila, Lise Hunault, Lana Saleh
      Pages 137-144
  4. Assessing the Levels and Spatial Distribution of DNA Modifications Using Antibody-Based Approaches

    1. Front Matter

      Pages 145-145
    2. Immunohistochemical Detection of Modified Cytosine Bases in Rodent Brain

      • Marcus D. Wallis, Rebecca C. Trueman
      Pages 183-191

About this book

This book provides an overview of methods and experimental protocols that are currently used to analyze the presence and abundance of non-canonical DNA nucleotides in different biological systems. Focusing particularly on the newly discovered and less studied DNA modifications that are enzymatically produced and are likely to play specific roles in various biological processes, the volume explores chromatography- and mass spectrometry-based techniques for the detection and quantification of DNA modifications, antibody-based approaches to study their spatial distribution in different cells and tissues, and methods to analyze their genomic distribution with the help of bioinformatics tools that interrogate the corresponding datasets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, DNA Modifications: Methods and Protocols serves as an ideal guide to research scientists and PhD students in this rapidly developing discipline, and, thus, will ultimately contribute to deciphering the roles of non-canonical DNA nucleotides in different biological systems.

Editors and Affiliations

  • Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham, UK

    Alexey Ruzov

  • School of Life Sciences, Queen’s Medical Centre, University of Nottingham, Nottingham, UK

    Martin Gering

Bibliographic Information

  • Book Title: DNA Modifications

  • Book Subtitle: Methods and Protocols

  • Editors: Alexey Ruzov, Martin Gering

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0876-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0875-3Published: 22 August 2020

  • Softcover ISBN: 978-1-0716-0878-4Published: 22 August 2021

  • eBook ISBN: 978-1-0716-0876-0Published: 21 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 495

  • Number of Illustrations: 27 b/w illustrations, 70 illustrations in colour

  • Topics: Biological Techniques, Genetics and Genomics

Buy it now

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 179.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 299.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access