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Testicular Germ Cell Tumors

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Aditya Bagrodia

    1. Department of Urology, UT Southwestern Medical Center, Dallas, USA

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  2. James F. Amatruda

    1. Cancer and Blood Disease Institute, Children’s Hospital Los Angeles, Departments of Pediatrics and Medicine, Keck School of Medicine, University of Southern California, Los Angeles, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2195)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Basic Histopathologic Assessment of Germ Cell Tumors for Clinic and Research

    • Payal Kapur, Dinesh Rakheja
    Pages 1-11

  3. Clinical Applications of Immunohistochemistry in Germ Cell Tumors in Men

    • Franto Francis, Ming Zhou
    Pages 13-29

  4. Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection

    • Liang Cheng, Steven A. Mann, Antonio Lopez-Beltran, Michal Chovanec, Matteo Santoni, Mingsheng Wang et al.
    Pages 31-47

  5. Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors

    • Liang Cheng, Darrell D. Davidson, Rodolfo Montironi, Mingsheng Wang, Antonio Lopez-Beltran, Timothy A. Masterson et al.
    Pages 49-63

  6. Germ Cell Tumor Cell Culture Techniques

    • John T. Lafin, James F. Amatruda, Aditya Bagrodia
    Pages 65-76

  7. Three-Dimensional Cultivation of Germ Cell Cancer Cell Lines as Hanging Drops

    • Margaretha A. Skowron, Meike M. Watolla, Daniel Nettersheim
    Pages 77-83

  8. Cultivation of Testicular Germ Cell Cancer Cell Lines and Establishment of Gene-Edited Subclones Using CRISPR/Cas9

    • Sina Jostes, Daniel Nettersheim, Simon Schneider, Hubert Schorle
    Pages 85-97

  9. Evaluation of Chemotherapeutic Drugs for Treatment of (Cisplatin-Resistant) Germ Cell Cancer Cell Lines

    • Margaretha A. Skowron, Michèle J. Hoffmann, Meike M. Watolla, Daniel Nettersheim
    Pages 99-111

  10. Assessing Homologous Recombination and Interstrand Cross-Link Repair in Embryonal Carcinoma Testicular Germ Cell Tumor Cell Lines

    • Francesca Cavallo, Cinzia Caggiano, Maria Jasin, Marco Barchi
    Pages 113-123

  11. Production and Analysis of Human Primordial Germ Cell–Like Cells

    • Shino Mitsunaga, Keiko Shioda, Jacob H. Hanna, Kurt J. Isselbacher, Toshi Shioda
    Pages 125-145

  12. A Genetically Engineered Mouse Model of Malignant Testicular Germ Cell Tumors

    • Amy M. Lyndaker, Timothy M. Pierpont, Amanda R. Loehr, Robert S. Weiss
    Pages 147-165

  13. Targeted Methylation Analyses: From Bisulfite Treatment to Quantification

    • João Lobo, Ad J. M. Gillis, Leendert H. J. Looijenga
    Pages 167-180

  14. Integrated Analysis of Germ Cell Tumors

    • Alok Tewari, Eliezer Van Allen
    Pages 181-187

  15. Use of Genomewide Association Studies to Evaluate Genetic Predisposition to Testicular Germ Cell Tumors

    • Anthony J. Hooten, Erica Langer, Jenny N. Poynter
    Pages 189-223

  16. A Circulating MicroRNA Panel for Malignant Germ Cell Tumor Diagnosis and Monitoring

    • Matthew J. Murray, Cinzia G. Scarpini, Nicholas Coleman
    Pages 225-243

  17. Detection of Circulating Tumor Cells (CTCs) in Patients with Testicular Germ Cell Tumors

    • Paulina Nastały, Friedemann Honecker, Klaus Pantel, Sabine Riethdorf
    Pages 245-261

  18. Developing and Using a Data Commons for Understanding the Molecular Characteristics of Germ Cell Tumors

    • Bo Ci, Shin-Yi Lin, Bo Yao, Danni Luo, Lin Xu, Mark Krailo et al.
    Pages 263-275

  19. Correction to: Germ Cell Tumor Cell Culture Techniques

    • John T. Lafin, James F. Amatruda, Aditya Bagrodia
    Pages C1-C1

  20. Back Matter

    Pages 277-278
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Keywords

About this book

This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics such as clinical applications of immunohistochemistry in GCTs in men; fluorescence in situ hybridization (FISH) detection of chromosomal 12p anomalies in testicular GCTs; evaluation of chemotherapeutic drugs for treatment of (cisplatin-resistant) germ cell cancer cell lines; targeted methylation analyses; and circulating microRNA panel for malignant GCT diagnosis and monitoring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Testicular Germ Cell Tumors: Methods and Protocols provides valuable information that is useful for both novice and expert GCT researchers interested in learning more about this field. 


Editors and Affiliations

  • Department of Urology, UT Southwestern Medical Center, Dallas, USA

    Aditya Bagrodia

  • Cancer and Blood Disease Institute, Children’s Hospital Los Angeles, Departments of Pediatrics and Medicine, Keck School of Medicine, University of Southern California, Los Angeles, USA

    James F. Amatruda

Bibliographic Information

  • Book Title: Testicular Germ Cell Tumors

  • Book Subtitle: Methods and Protocols

  • Editors: Aditya Bagrodia, James F. Amatruda

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0860-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0859-3Published: 28 August 2020

  • Softcover ISBN: 978-1-0716-0862-3Published: 28 August 2021

  • eBook ISBN: 978-1-0716-0860-9Published: 27 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 278

  • Number of Illustrations: 5 b/w illustrations, 44 illustrations in colour

  • Topics: Developmental Biology, Cancer Research, Cell Biology

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