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Channelrhodopsin

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Robert E. Dempski

    1. Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2191)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
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  2. Basic Science

    1. Front Matter

      Pages 1-1
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    2. Molecular Dynamics Simulations of Channelrhodopsin Chimera, C1C2

      • Monika R. VanGordon
      Pages 3-15

    3. Computing Potential of the Mean Force Profiles for Ion Permeation Through Channelrhodopsin Chimera, C1C2

      • Chad Priest, Monika R. VanGordon, Caroline Rempe, Mangesh I. Chaudhari, Mark J. Stevens, Steve Rick et al.
      Pages 17-28

    4. Nanodisc Reconstitution of Channelrhodopsins Heterologously Expressed in Pichia pastoris for Biophysical Investigations

      • Maria Walter, Ramona Schlesinger
      Pages 29-48

    5. Characterizing Channelrhodopsin Channel Properties Via Two-Electrode Voltage Clamp and Kinetic Modeling

      • Lindsey Prignano, Lauren Herchenroder, Robert E. Dempski
      Pages 49-63

  3. Cells

    1. Front Matter

      Pages 65-65
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    2. Charge Transport by Light-Activated Rhodopsins Determined by Electrophysiological Recordings

      • Tamara Hussein, Christian Bamann
      Pages 67-84

    3. Probing Channelrhodopsin Electrical Activity in Algal Cell Populations

      • Oleg A. Sineshchekov, Elena G. Govorunova, John L. Spudich
      Pages 85-96

    4. Two-Photon Optogenetic Stimulation of Drosophila Neurons

      • Mehmet Fişek, James M. Jeanne
      Pages 97-108

    5. Probing Synaptic Signaling with Optogenetic Stimulation and Genetically Encoded Calcium Reporters

      • Gabriel B. Borja, Himali Shroff, Hansini Upadhyay, Pin W. Liu, Valeriya Baru, Yung-Chih Cheng et al.
      Pages 109-134

    6. Optogenetics to Interrogate Neuron-Glia Interactions in Pups and Adults

      • Chloé Habermacher, Blandine Manot-Saillet, Domiziana Ortolani, Fernando C. Ortiz, María Cecilia Angulo
      Pages 135-149

    7. Chronic Optogenetic Pacing of Human-Induced Pluripotent Stem Cell-Derived Engineered Cardiac Tissues

      • Marc Dwenger, William J. Kowalski, Hidetoshi Masumoto, Takeichiro Nakane, Bradley B. Keller
      Pages 151-169

  4. Animals

    1. Front Matter

      Pages 171-171
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    2. Patterned Optogenetic Stimulation Using a DMD Projector

      • Aanchal Bhatia, Sahil Moza, Upinder S. Bhalla
      Pages 173-188

    3. Selecting Channelrhodopsin Constructs for Optimal Visual Restoration in Differing Light Conditions

      • Tushar H. Ganjawala, Zhuo-Hua Pan
      Pages 189-199

    4. Recording Channelrhodopsin-Evoked Field Potentials and Startle Responses from Larval Zebrafish

      • Yagmur Idil Ozdemir, Christina A. Hansen, Mohamed A. Ramy, Eileen L. Troconis, Lauren D. McNeil, Josef G. Trapani
      Pages 201-220

    5. Automated Functional Screening for Modulators of Optogenetically Activated Neural Responses in Living Organisms

      • Ross C. Lagoy, Eric Larsen, Dirk R. Albrecht
      Pages 221-233

    6. Optogenetic Interrogation of ChR2-Expressing GABAergic Interneurons After Transplantation into the Mouse Brain

      • Muhammad N. Arshad, Gloster B. Aaron, Janice R. Naegele
      Pages 235-259

    7. Application of Targeting-Optimized Chronos for Stimulation of the Auditory Pathway

      • Antoine Tarquin Huet, Vladan Rankovic
      Pages 261-285
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Keywords

About this book

This book merges approaches in understanding the function of the light-gated ion channels known as channelrhodopsin together with methods addressing how channelrhodopsins can be used to address biomedical questions on a cellular or organismal level. Since the first molecular identification of channelrhodopsins, a broad range of tools have been created and new approaches developed to both better understand the molecular determinants of channelrhodopsin function as well as to use these and homologous proteins from a variety of species as tools to better understand physiological processes, which this volume addresses. Additionally, channelrhodopsins have become instrumental as a potential treatment for disease states. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Channelrhodopsin: Methods and Protocols provides a resource for those interested in honing their current expertise in this vital area of study as well as potentially branching out into new directions.

Editors and Affiliations

  • Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, USA

    Robert E. Dempski

Bibliographic Information

  • Book Title: Channelrhodopsin

  • Book Subtitle: Methods and Protocols

  • Editors: Robert E. Dempski

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0830-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0829-6Published: 01 September 2020

  • Softcover ISBN: 978-1-0716-0832-6Published: 01 September 2021

  • eBook ISBN: 978-1-0716-0830-2Published: 31 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 405

  • Number of Illustrations: 18 b/w illustrations, 99 illustrations in colour

  • Topics: Protein Science, Microbiology

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