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Immunometabolism

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Suresh Mishra

    1. Faculty of Health Sciences, Department of Internal Medicine, University of Manitoba, Winnipeg, Canada

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2184)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Single-Cell Transcriptomics of Immune Cells: Cell Isolation and cDNA Library Generation for scRNA-Seq

    • Janilyn Arsenio
    Pages 1-18

  3. Monitoring Lactate Dynamics in Individual Macrophages with a Genetically Encoded Probe

    • Felipe Baeza-Lehnert, Carlos A. Flores, Anita Guequén, L. Felipe Barros
    Pages 19-30

  4. The Conjugation of Antibodies for the Simultaneous Detection of Surface Proteins and Transcriptome Analysis at a Single-Cell Level

    • Iivari Kleino, Eliisa Kekäläinen, Tapio Lönnberg
    Pages 31-45

  5. Cellular Fatty Acid Analysis in Macrophage Using Stable Isotope Labeling

    • Kevin J. Williams, Steven J. Bensinger
    Pages 47-60

  6. Proteomics Network Analysis of Polarized Macrophages

    • Jayanta K. Chakrabarty, Abu Hena Mostafa Kamal, A. D. A. Shahinuzzaman, Saiful M. Chowdhury
    Pages 61-75

  7. The Purification and Characterization of Exosomes from Macrophages

    • Eran Fridman, Lana Ginini, Ziv Gil, Neta Milman
    Pages 77-90

  8. Isolation and Characterization of T Lymphocyte-Exosomes Using Mass Spectrometry

    • Inbar Azoulay-Alfaguter, Adam Mor
    Pages 91-102

  9. Analysis of Immune-Tumor Cell Interactions Using a 3D Co-culture Model

    • Tanya N. Augustine
    Pages 103-110

  10. Studying Adipocyte and Immune Cell Cross Talk Using a Co-culture System

    • Jennifer M. Monk, Danyelle M. Liddle, Amber L. Hutchinson, Lindsay E. Robinson
    Pages 111-130

  11. A Gene Expression Analysis of M1 and M2 Polarized Macrophages

    • Nour Eissa, Hayam Hussein, Jean-Eric Ghia
    Pages 131-144

  12. Simultaneous, Quantitative Characterization of Protein ADP-Ribosylation and Protein Phosphorylation in Macrophages

    • Casey M. Daniels, Arthur Nuccio, Pauline R. Kaplan, Aleksandra Nita-Lazar
    Pages 145-160

  13. The Analysis of Mycobacterium tuberculosis-Induced Bioenergetic Changes in Infected Macrophages Using an Extracellular Flux Analyzer

    • Bridgette M. Cumming, Vineel P. Reddy, Adrie J. C. Steyn
    Pages 161-184

  14. Analyzing the Metabolic Phenotype of Bone Marrow-Derived Dendritic Cells by Assessing Their Oxygen Consumption and Extracellular Acidification

    • Hsi-Ju Wei, John J. Letterio, Tej K. Pareek
    Pages 185-196

  15. The Evaluation of Mitochondrial Membrane Potential Using Fluorescent Dyes or a Membrane-Permeable Cation (TPP+) Electrode in Isolated Mitochondria and Intact Cells

    • João S. Teodoro, Ivo F. Machado, Ana C. Castela, Anabela P. Rolo, Carlos M. Palmeira
    Pages 197-213

  16. The Multiparametric Analysis of Mitochondrial Dynamics in T Cells from Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs)

    • Jesse J. R. Masson, Matias Ostrowski, Gabriel Duette, Man K. S. Lee, Andrew J. Murphy, Suzanne M. Crowe et al.
    Pages 215-224

  17. The Measurement of Whole-Body Glucose Homeostasis in Mice

    • Yang Xin Zi Xu, Sudharsana R. Ande, Suresh Mishra
    Pages 225-231

  18. Immunometabolism and Its Potential to Improve the Current Limitations of Immunotherapy

    • Andrew D. Sheppard, Joanne Lysaght
    Pages 233-263

  19. Sex Differences in Immunometabolism: An Unexplored Area

    • Suresh Mishra, Geetika Bassi, Yang Xin Zi Xu
    Pages 265-271

  20. Isolation and Preparation of Bone Marrow-Derived Immune Cells for Metabolic Analysis

    • Nnamdi M. Ikeogu, Chidalu A. Edechi, Gloria N. Akaluka, Aida Feiz-Barazandeh, Jude E. Uzonna
    Pages 273-280
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Keywords

About this book

This detailed book showcases the tremendous effort and progress made in developing techniques and protocols for the study of immunometabolism, and in utilizing recent technological advances for probing and manipulating adipose and immune cells, and subsequently, their functions and immunometabolic consequences. Written by experts in the field, many chapters use macrophages as a model immune cell type, due to their prominence in the innate immune system and the exhaustive study of their traits. Protocols using adipocytes, dendritic cells, and T cells as model cell lines, as well as measurement of glucose metabolism at the systemic level, have also been included. Written for the highly successful Methods in Molecular Biology book series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritativeand practical, Immunometabolism: Methods and Protocols serves as a vital guide for researchers working at the important interface of immunology and metabolism.

Editors and Affiliations

  • Faculty of Health Sciences, Department of Internal Medicine, University of Manitoba, Winnipeg, Canada

    Suresh Mishra

Bibliographic Information

  • Book Title: Immunometabolism

  • Book Subtitle: Methods and Protocols

  • Editors: Suresh Mishra

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0802-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0801-2Published: 19 August 2020

  • Softcover ISBN: 978-1-0716-0804-3Published: 19 August 2021

  • eBook ISBN: 978-1-0716-0802-9Published: 17 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 284

  • Number of Illustrations: 10 b/w illustrations, 48 illustrations in colour

  • Topics: Immunology, Chemistry/Food Science, general, Physiology

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