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Cryopreservation and Freeze-Drying Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Willem F. Wolkers

    1. Unit for Reproductive Medicine—Clinic for Horses, Biostabilization Laboratory—Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, University of Veterinary Medicine Hannover, Hannover, Germany, University of Veterinary Medicine Hannover, Hannover, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Harriëtte Oldenhof

    1. Unit for Reproductive Medicine—Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2180)

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Table of contents (41 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Fundamental Aspects of Cryopreservation and Freeze-Drying

    1. Front Matter

      Pages 1-1
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    2. Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues

      • Willem F. Wolkers, Harriëtte Oldenhof
      Pages 3-25

    3. Principles of Ice-Free Cryopreservation by Vitrification

      • Gregory M. Fahy, Brian Wowk
      Pages 27-97

    4. The Principles of Freeze-Drying and Application of Analytical Technologies

      • Kevin R. Ward, Paul Matejtschuk
      Pages 99-127

    5. Mathematical Modeling and Optimization of Cryopreservation in Single Cells

      • James D. Benson
      Pages 129-172

    6. Mathematical Modeling of Protectant Transport in Tissues

      • Ross M. Warner, Adam Z. Higgins
      Pages 173-188

  3. Technologies and Methods to Study Freezing and Drying

    1. Front Matter

      Pages 189-189
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    2. Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation

      • Peter Kilbride, Julie Meneghel
      Pages 191-201Open Access

    3. Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of Biologics

      • Shangping Wang, Susan Trammell, Gloria D. Elliott
      Pages 203-220

    4. High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kinetics

      • Jens O. M. Karlsson
      Pages 221-269

    5. Use of Ice Recrystallization Inhibition Assays to Screen for Compounds That Inhibit Ice Recrystallization

      • Anna A. Ampaw, August Sibthorpe, Robert N. Ben
      Pages 271-283

    6. DSC Analysis of Thermophysical Properties for Biomaterials and Formulations

      • Wendell Q. Sun
      Pages 285-302

    7. Osmometric Measurements of Cryoprotective Agent Permeation into Tissues

      • Kezhou Wu, Leila Laouar, Nadia Shardt, Janet A. W. Elliott, Nadr M. Jomha
      Pages 303-315

    8. Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes

      • Ariadna Corral, Alberto Olmo, Ramón Risco
      Pages 317-330

    9. Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological Research

      • Willem F. Wolkers, Harriëtte Oldenhof
      Pages 331-349

    10. Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements

      • Guanglin Yu, Rui Li, Allison Hubel
      Pages 351-361

  4. Cryopreservation and Freeze-Drying Protocols

    1. Front Matter

      Pages 363-363
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    2. Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm

      • Harriëtte Oldenhof, Willem F. Wolkers, Harald Sieme
      Pages 365-377

    3. Cryopreservation of Avian Semen

      • Henri Woelders
      Pages 379-399
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Keywords

About this book

This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.

The chapter “Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.


Editors and Affiliations

  • Unit for Reproductive Medicine—Clinic for Horses, Biostabilization Laboratory—Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, University of Veterinary Medicine Hannover, Hannover, Germany, University of Veterinary Medicine Hannover, Hannover, Germany

    Willem F. Wolkers

  • Unit for Reproductive Medicine—Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany

    Harriëtte Oldenhof

Bibliographic Information

  • Book Title: Cryopreservation and Freeze-Drying Protocols

  • Editors: Willem F. Wolkers, Harriëtte Oldenhof

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0783-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0782-4Published: 15 August 2020

  • Softcover ISBN: 978-1-0716-0785-5Published: 12 May 2022

  • eBook ISBN: 978-1-0716-0783-1Published: 14 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 4

  • Number of Pages: XVI, 742

  • Number of Illustrations: 52 b/w illustrations, 104 illustrations in colour

  • Topics: Biotechnology

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