Methods in Molecular Biology

Cryopreservation and Freeze-Drying Protocols

Editors: Wolkers, Willem F., Oldenhof, Harriëtte (Eds.)

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About this book

This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.

The chapter “Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Table of contents (41 chapters)

Table of contents (41 chapters)
  • Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues

    Pages 3-25

    Wolkers, Willem F. (et al.)

  • Principles of Ice-Free Cryopreservation by Vitrification

    Pages 27-97

    Fahy, Gregory M. (et al.)

  • The Principles of Freeze-Drying and Application of Analytical Technologies

    Pages 99-127

    Ward, Kevin R. (et al.)

  • Mathematical Modeling and Optimization of Cryopreservation in Single Cells

    Pages 129-172

    Benson, James D.

  • Mathematical Modeling of Protectant Transport in Tissues

    Pages 173-188

    Warner, Ross M. (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-0716-0783-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-0716-0782-4
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $159.99
price for USA in USD
  • Customers within the U.S. and Canada please contact Customer Service at +1-800-777-4643, Latin America please contact us at +1-212-460-1500 (24 hours a day, 7 days a week). Pre-ordered printed titles are excluded from promotions.
  • Due: August 29, 2021
  • ISBN 978-1-0716-0785-5
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Shipping restrictions
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Bibliographic Information

Bibliographic Information
Book Title
Cryopreservation and Freeze-Drying Protocols
Editors
  • Willem F. Wolkers
  • Harriëtte Oldenhof
Series Title
Methods in Molecular Biology
Series Volume
2180
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0783-1
DOI
10.1007/978-1-0716-0783-1
Hardcover ISBN
978-1-0716-0782-4
Softcover ISBN
978-1-0716-0785-5
Series ISSN
1064-3745
Edition Number
4
Number of Pages
XVI, 742
Number of Illustrations
52 b/w illustrations, 104 illustrations in colour
Topics