Read While You Wait - Get immediate ebook access, if available*, when you order a print book

Methods in Molecular Biology

Caveolae

Methods and Protocols

Editors: Blouin, Cedric (Ed.)

Free Preview
  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-0716-0732-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-0716-0731-2
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
About this book

This volume explores techniques used to study either the structure or the functions of caveolae and their components in several normal and pathophysiological situations. The chapters in this book cover topics such as selective visualization of caveolae by electron microscopy techniques; spatiotemporal analysis of caveolae dynamics and mechanics using live cell fluorescence microscopy; in vitro reconstitution such as liposomes and GPMVs as tools to study caveolin- and cavin-interacting partners; pulling of tethers from the cell plasma membrane using optical tweezers; and immunofluorescence-based analysis of caveolin-3 in the diagnostic management of neuromuscular diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, Caveolae: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in discovering new roles or regulations of formed caveolae, and proteins composing their coat in various model organisms.  


Table of contents (18 chapters)

Table of contents (18 chapters)
  • Selective Visualization of Caveolae by TEM Using APEX2

    Pages 1-10

    Ludwig, Alexander

  • Freeze-Fracture Replica Immunolabeling of Cryopreserved Membrane Compartments, Cultured Cells and Tissues

    Pages 11-25

    Seemann, Eric (et al.)

  • Analysis of Caveolin in Primary Cilia

    Pages 27-41

    Morthorst, Stine K. (et al.)

  • Method for Efficient Observation of Caveolin-1 in Plasma Membrane by Microscopy Imaging Analysis

    Pages 43-52

    Yamaguchi, Tomoya (et al.)

  • Quantitative Image Analysis of the Spatial Organization and Mobility of Caveolin Aggregates at the Plasma Membrane

    Pages 53-62

    Hirama, Takashi (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-0716-0732-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-0716-0731-2
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Caveolae
Book Subtitle
Methods and Protocols
Editors
  • Cedric Blouin
Series Title
Methods in Molecular Biology
Series Volume
2169
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0732-9
DOI
10.1007/978-1-0716-0732-9
Hardcover ISBN
978-1-0716-0731-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 218
Number of Illustrations
9 b/w illustrations, 37 illustrations in colour
Topics

*immediately available upon purchase as print book shipments may be delayed due to the COVID-19 crisis. ebook access is temporary and does not include ownership of the ebook. Only valid for books with an ebook version. Springer Reference Works are not included.