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Methods in Molecular Biology

Ribozymes

Methods and Protocols

Editors: Scarborough, Robert, Gatignol, Anne (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
price for USA in USD
  • ISBN 978-1-0716-0716-9
  • Digitally watermarked, DRM-free
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Hardcover $199.99
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About this book

This volume provides protocols designed to study the function and the structure of diverse ribozymes. Chapters guide readers through different techniques to identify and characterize new ribozymes and methods to use ribozymes to alter the function of CRISPR-based guide RNAs, AgoshRNAs and aptamers or to study RNA capping and long non-coding RNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Ribozymes: Methods and Protocols aims to help in accelerating ribozyme research and inspiring others to develop new methods to study ribozyme structure and function.


Table of contents (17 chapters)

Table of contents (17 chapters)
  • Conjugation as a Highly Sensitive Assay to Study Group II Intron Splicing In Vivo

    Pages 3-11

    LaRoche-Johnston, Félix (et al.)

  • Co-transcriptional Analysis of Self-Cleaving Ribozymes and Their Ligand Dependence

    Pages 13-24

    Passalacqua, Luiz F. M. (et al.)

  • Cloning and Detection of Genomic Retrozymes and Their circRNA Intermediates

    Pages 27-44

    Cervera, Amelia (et al.)

  • Demonstration of a Ribozyme in Epsilon Domain of Hepatitis B Virus RNA

    Pages 45-59

    Chakraborty, Dibyajnan (et al.)

  • In Vitro Selection of Varkud Satellite Ribozyme Variants that Cleave a Modified Stem-Loop Substrate

    Pages 61-77

    Dagenais, Pierre (et al.)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-0716-0716-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Ribozymes
Book Subtitle
Methods and Protocols
Editors
  • Robert Scarborough
  • Anne Gatignol
Series Title
Methods in Molecular Biology
Series Volume
2167
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0716-9
DOI
10.1007/978-1-0716-0716-9
Hardcover ISBN
978-1-0716-0715-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 304
Number of Illustrations
29 b/w illustrations, 43 illustrations in colour
Topics