- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Buy this book
- About this book
-
This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology.
- Table of contents (27 chapters)
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Tagging and Application of RNA Probes for Sequence-Specific Visualization of RNAs by Fluorescent In Situ Hybridization
Pages 3-21
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Quantitative Fluorescence In Situ Hybridization Detection of Plant mRNAs with Single-Molecule Resolution
Pages 23-33
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Visualization of Endoplasmic Reticulum-Associated mRNA in Mammalian Cells
Pages 35-49
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Simultaneous Detection of mRNA and Protein in S. cerevisiae by Single-Molecule FISH and Immunofluorescence
Pages 51-69
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Development and Applications of Fluorogen/Light-Up RNA Aptamer Pairs for RNA Detection and More
Pages 73-102
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Table of contents (27 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- RNA Tagging
- Book Subtitle
- Methods and Protocols
- Editors
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- Manfred Heinlein
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2166
- Copyright
- 2020
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-0712-1
- DOI
- 10.1007/978-1-0716-0712-1
- Hardcover ISBN
- 978-1-0716-0711-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIV, 490
- Number of Illustrations
- 14 b/w illustrations, 77 illustrations in colour
- Topics
