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  • © 2020

Kupffer Cells

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2164)

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eBook USD 109.00
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Softcover Book USD 139.99
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Hardcover Book USD 199.99
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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
  2. Kupffer Cell Isolation from Human Biopsies

    • Cecilia Morgantini
    Pages 11-13
  3. Kupffer Cell mRNA Sequencing

    • Emelie Barreby, Connie Xu
    Pages 27-44
  4. Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model

    • Qiushi Tang, Gwladys Gernoux, Yangfun Cheng, Terence Flotte, Christian Mueller
    Pages 75-85
  5. Kupffer Cell Characterization by Mass Cytometry

    • Camille Blériot, Shamin Li, Muhammad Faris Bin Mohd Kairi, Evan Newell, Florent Ginhoux
    Pages 87-99
  6. Enzymatic Quantification of Liver Lipids After Folch Extraction

    • Mirko E. Minniti, Osman Ahmed, Matteo Pedrelli
    Pages 101-108
  7. An Obesogenic Dietary Mouse Model of Nonalcoholic Steatohepatitis

    • Bader Zarrouki, Jeremie Boucher
    Pages 121-127
  8. Mouse Model of Alcoholic Steatohepatitis

    • Adeline Bertola
    Pages 145-157
  9. Back Matter

    Pages 159-160

About this book

This volume provides a complete overview of kupffer cell biology, from isolation to phenotyping. Chapters guide readers through methods and protocols on phenotyping, including RNA sequencing, Mass cytometry and 3D microscopy, as well as gene manipulation in vivo, and disease models from fatty liver disease to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

  

Authoritative and cutting-edge, Kupffer Cells: Methods and Protocols  aims to ensure successful investigation of this growing field. 

Editors and Affiliations

  • Department of Medicine, Integrated Cardio Metabolic Center, Karolinska Institutet, Huddinge, Sweden

    Myriam Aouadi, Valerio Azzimato

Bibliographic Information

  • Book Title: Kupffer Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Myriam Aouadi, Valerio Azzimato

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0704-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0703-9Published: 01 July 2020

  • Softcover ISBN: 978-1-0716-0706-0Published: 01 July 2021

  • eBook ISBN: 978-1-0716-0704-6Published: 30 June 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 160

  • Number of Illustrations: 10 b/w illustrations, 24 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access