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Methods in Molecular Biology

Dynamin Superfamily GTPases

Methods and Protocols

Editors: Ramachandran, Rajesh (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

This volume provides methods and protocols on dynamin superfamily GTPases. Chapters are divided into three parts; detailing expression, purification, initial biochemical steps, undescribed analytical techniques, and biophysical and cellular characterization of DSPs in membrane remodeling, fission and fusion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Dynamin Superfamily GTPases: Methods and Protocols to ensure successful results in the further study of this vital field.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Isolation and Analysis of Mitochondrial Fission Enzyme DNM1 from Saccharomyces cerevisiae

    Pages 3-15

    Kennedy, Nolan W. (et al.)

  • Purification of the Dynamin-Related Protein Vps1 Using Mammalian and Bacterial Expression Systems

    Pages 17-27

    Varlakhanova, Natalia V. (et al.)

  • A Single Common Protocol for the Expression and Purification of Soluble Mammalian DSPs from Escherichia coli

    Pages 31-40

    Stepanyants, Natalia (et al.)

  • Affinity Purification and Functional Characterization of Dynamin-Related Protein 1

    Pages 41-53

    Clinton, Ryan W. (et al.)

  • Purification and Characterization of MxB

    Pages 55-65

    Alvarez, Frances Joan D. (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-0716-0676-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-1-0716-0675-9
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Dynamin Superfamily GTPases
Book Subtitle
Methods and Protocols
Editors
  • Rajesh Ramachandran
Series Title
Methods in Molecular Biology
Series Volume
2159
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0676-6
DOI
10.1007/978-1-0716-0676-6
Hardcover ISBN
978-1-0716-0675-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 221
Number of Illustrations
20 b/w illustrations, 33 illustrations in colour
Topics

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