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Dynamin Superfamily GTPases

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Rajesh Ramachandran

    1. School of Medicine, Case Western Reserve University, Cleveland, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2159)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Yeast DSPs: Isolation and Biochemical Characterization

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Isolation and Analysis of Mitochondrial Fission Enzyme DNM1 from Saccharomyces cerevisiae

      • Nolan W. Kennedy, Lora K. Picton, R. Blake Hill
      Pages 3-15

    3. Purification of the Dynamin-Related Protein Vps1 Using Mammalian and Bacterial Expression Systems

      • Natalia V. Varlakhanova, Marijn G. J. Ford
      Pages 17-27

  3. Mammalian DSPs: Isolation and Biochemical Characterization

    1. Front Matter

      Pages 29-29
      Download chapter PDF

    2. A Single Common Protocol for the Expression and Purification of Soluble Mammalian DSPs from Escherichia coli

      • Natalia Stepanyants, Patrick J. Macdonald, Pooja Madan Mohan, Rajesh Ramachandran
      Pages 31-40

    3. Affinity Purification and Functional Characterization of Dynamin-Related Protein 1

      • Ryan W. Clinton, Brianna L. Bauer, Jason A. Mears
      Pages 41-53

    4. Purification and Characterization of MxB

      • Frances Joan D. Alvarez, Peijun Zhang
      Pages 55-65

    5. Purification of Farnesylated hGBP1 and Characterization of Its Polymerization and Membrane Binding

      • Linda Sistemich, Christian Herrmann
      Pages 67-81

  4. Biophysical and Cellular Characterization of DSPs

    1. Front Matter

      Pages 83-83
      Download chapter PDF

    2. Microscale Thermophoresis (MST ) as a Tool for Measuring Dynamin Superfamily Protein (DSP)–Lipid Interactions

      • Nikhil Bharambe, Rajesh Ramachandran
      Pages 85-92

    3. Nucleotide-Dependent Dimerization and Conformational Switching of Atlastin

      • John P. O’Donnell, Carolyn M. Kelly, Holger Sondermann
      Pages 93-113

    4. Analysis of Mitochondrial Membrane Fusion GTPase OPA1 Expressed by the Silkworm Expression System

      • Tadato Ban, Naotada Ishihara
      Pages 115-127

    5. Cell-Free Analysis of Mitochondrial Fusion by Fluorescence Microscopy

      • Nyssa Becker Samanas, Suzanne Hoppins
      Pages 129-140

    6. Electrophysiological Methods for Detection of Membrane Leakage and Hemifission by Dynamin 1

      • Pavel V. Bashkirov, Ksenia V. Chekashkina, Anna V. Shnyrova, Vadim A. Frolov
      Pages 141-162

    7. Integrating Optical and Electrochemical Approaches to Assess the Actions of Dynamin at the Fusion Pore

      • Katherine A. Smith, Emily R. Prantzalos, Arun Anantharam
      Pages 163-178

    8. Cellular Assays for Measuring Dynamin Activity in Muscle Cells

      • Jessica Laiman, Ya-Wen Liu
      Pages 179-187

    9. Measuring Drp1 Activity in Mitochondrial Fission In Vivo

      • Di Hu, Xin Qi
      Pages 189-195

    10. Quantifying Drp1-Mediated Mitochondrial Fission by Immunostaining in Fixed Cells

      • Di Hu, Xin Qi
      Pages 197-204

    11. Imaging Dynamin-Related Protein 1 (Drp1)-Mediated Mitochondrial Fission in Living Cells

      • Felipe Montecinos-Franjola, Rajesh Ramachandran
      Pages 205-217
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Keywords

About this book

This volume provides methods and protocols on dynamin superfamily GTPases. Chapters are divided into three parts; detailing expression, purification, initial biochemical steps, undescribed analytical techniques, and biophysical and cellular characterization of DSPs in membrane remodeling, fission and fusion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Dynamin Superfamily GTPases: Methods and Protocols to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • School of Medicine, Case Western Reserve University, Cleveland, USA

    Rajesh Ramachandran

Bibliographic Information

  • Book Title: Dynamin Superfamily GTPases

  • Book Subtitle: Methods and Protocols

  • Editors: Rajesh Ramachandran

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0676-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0675-9Published: 12 June 2020

  • Softcover ISBN: 978-1-0716-0678-0Published: 12 June 2021

  • eBook ISBN: 978-1-0716-0676-6Published: 11 June 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 221

  • Number of Illustrations: 20 b/w illustrations, 33 illustrations in colour

  • Topics: Chemistry/Food Science, general

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