Methods in Molecular Biology

Pollen and Pollen Tube Biology

Methods and Protocols

Editors: Geitmann, Anja (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-0716-0672-8
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About this book

This volume explores a collection of experimental techniques used to investigate different aspects of pollen development and function, including its role in reaching the ovule and delivering the two sperm cells. The techniques discussed range from basic methodology to cultivate pollen in vitro to the sophisticated experiments involving micromanipulation, Lab-on-Chip technology, or high-end imaging. The chapters in this book cover topics such as pollen grain counting using a cell counter; restricted pollination for tracing individual pollen tubes in a pistil; obtaining mutant pollen for phenotypic analysis and pollen tube dual-staining; analyzing intracellular gradients in pollen tubes; and measuring exocytosis rate in Arabidopsis pollen tube using corrected fluorescence recovery after photoconversion (cFRAPc) technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Pollen and Pollen Tube Biology: Methods and Protocols is a valuable resource for novice and expert scientists interested in learning more about the field of plant reproduction. 


Table of contents (22 chapters)

Table of contents (22 chapters)
  • Pollen Grain Counting Using a Cell Counter

    Pages 1-11

    Kakui, Hiroyuki (et al.)

  • A Toolkit for Teasing Apart the Early Stages of Pollen–Stigma Interactions in Arabidopsis thaliana

    Pages 13-28

    Lee, Hyun Kyung (et al.)

  • Decapitation Crosses to Test Pollen Fertility Mutations for Defects in Stigma-Style Penetration

    Pages 29-40

    Weigand, Chrystle (et al.)

  • Isolation of the Pistil-Stimulated Pollen Tube Secretome

    Pages 41-72

    Hafidh, Said (et al.)

  • Restricted Pollination for Tracing Individual Pollen Tubes in a Pistil

    Pages 73-81

    Takahashi, Taro (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-0716-0672-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Pollen and Pollen Tube Biology
Book Subtitle
Methods and Protocols
Editors
  • Anja Geitmann
Series Title
Methods in Molecular Biology
Series Volume
2160
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0672-8
DOI
10.1007/978-1-0716-0672-8
Hardcover ISBN
978-1-0716-0671-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 332
Number of Illustrations
7 b/w illustrations, 90 illustrations in colour
Topics