Pollen and Pollen Tube Biology
Methods and Protocols
Editors: Geitmann, Anja (Ed.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume explores a collection of experimental techniques used to investigate different aspects of pollen development and function, including its role in reaching the ovule and delivering the two sperm cells. The techniques discussed range from basic methodology to cultivate pollen in vitro to the sophisticated experiments involving micromanipulation, Lab-on-Chip technology, or high-end imaging. The chapters in this book cover topics such as pollen grain counting using a cell counter; restricted pollination for tracing individual pollen tubes in a pistil; obtaining mutant pollen for phenotypic analysis and pollen tube dual-staining; analyzing intracellular gradients in pollen tubes; and measuring exocytosis rate in Arabidopsis pollen tube using corrected fluorescence recovery after photoconversion (cFRAPc) technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Pollen and Pollen Tube Biology: Methods and Protocols is a valuable resource for novice and expert scientists interested in learning more about the field of plant reproduction.
- Table of contents (22 chapters)
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Pollen Grain Counting Using a Cell Counter
Pages 1-11
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A Toolkit for Teasing Apart the Early Stages of Pollen–Stigma Interactions in Arabidopsis thaliana
Pages 13-28
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Decapitation Crosses to Test Pollen Fertility Mutations for Defects in Stigma-Style Penetration
Pages 29-40
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Isolation of the Pistil-Stimulated Pollen Tube Secretome
Pages 41-72
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Restricted Pollination for Tracing Individual Pollen Tubes in a Pistil
Pages 73-81
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Table of contents (22 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Pollen and Pollen Tube Biology
- Book Subtitle
- Methods and Protocols
- Editors
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- Anja Geitmann
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2160
- Copyright
- 2020
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-0672-8
- DOI
- 10.1007/978-1-0716-0672-8
- Hardcover ISBN
- 978-1-0716-0671-1
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIII, 332
- Number of Illustrations
- 7 b/w illustrations, 90 illustrations in colour
- Topics
