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Methods in Molecular Biology
cover

Molecular Dermatology

Methods and Protocols

Editors: Botchkareva, ​Natalia V., Westgate, Gillian E. (Eds.)

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $149.00
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  • ISBN 978-1-07-160648-3
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Hardcover $199.99
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  • ISBN 978-1-07-160647-6
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About this book

This volume delivers the most important laboratory techniques that underlie dermatological investigation, including in situin vitroex vivo, and in vivo models of skin, its appendages (hair follicles, sebaceous and sweat glands), and immune cells. Also included are unique techniques that unravel epigenetic mechanisms in skin and hair follicle cells, such as gene organization in the nucleus and gene expression regulation by the non-coding transcriptome, which are important recent developments in investigative dermatology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Molecular Dermatology: Methods and Protocols serves as an essential resource for researchers working in the areas of fundamental as well as applied dermatological science.

Table of contents (22 chapters)

Table of contents (22 chapters)
  • Isolation of Epidermal Keratinocytes from Human Skin: The Scratch-Wound Assay for Assessment of Epidermal Keratinocyte Migration

    Pages 1-12

    Castellano-Pellicena, Irene (et al.)

  • Isolation of Different Dermal Fibroblast Populations from the Skin and the Hair Follicle

    Pages 13-22

    Williams, Rachael (et al.)

  • Isolation of Epidermal and Hair Follicle Melanocytes

    Pages 23-32

    Baker, Richard (et al.)

  • Isolation and Culture of Human Skin Mast Cells

    Pages 33-43

    Siiskonen, Hanna (et al.)

  • Organotypic 3D Skin Models: Human Epidermal Equivalent Cultures from Primary Keratinocytes and Immortalized Keratinocyte Cell Lines

    Pages 45-61

    Rikken, Gijs (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-07-160648-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-07-160647-6
  • with online files
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Molecular Dermatology
Book Subtitle
Methods and Protocols
Editors
  • ​Natalia V. Botchkareva
  • Gillian E. Westgate
Series Title
Methods in Molecular Biology
Series Volume
2154
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-07-160648-3
DOI
10.1007/978-1-0716-0648-3
Hardcover ISBN
978-1-07-160647-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 278
Number of Illustrations
6 b/w illustrations, 69 illustrations in colour
Topics

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