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Methods in Molecular Biology

Homologous Recombination

Methods and Protocols

Editors: Aguilera, Andres, Carreira, Aura (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-0716-0644-5
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About this book

This volume explores homologous recombination’s (HR) essential role in meiotic and somatic cells. It discusses the analysis of different steps of the HR process from the genetic, molecular biology, and cell biology perspectives. Using a variety of model systems, chapters in this book cover topics such as the genome-wide mapping of DNA double-strand breaks (DSB); analysis of DNA-end resection and recombination intermediates by gel electrophoresis and southern blotting; cell-based monitoring of HR activity in meiotic and mitotic cells; in vitro analysis of branch migration, DNA strand exchange and Holliday junction resolution; or super resolution imaging of HR repair at collapsed replication forks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Homologous Recombination: Methods and Protocols is a valuable resource that uses both classical and more recent approaches to answer questions on the HR mechanism. It is a useful tool for scientists working on the field of genome integrity, as well as those working in cancer biology and DNA repair. 


Table of contents (36 chapters)

Table of contents (36 chapters)
  • Detection of DNA Double-Strand Breaks by γ-H2AX Immunodetection

    Pages 1-8

    Barroso, Sonia I. (et al.)

  • END-seq: An Unbiased, High-Resolution, and Genome-Wide Approach to Map DNA Double-Strand Breaks and Resection in Human Cells

    Pages 9-31

    Wong, Nancy (et al.)

  • Resection of a DNA Double-Strand Break by Alkaline Gel Electrophoresis and Southern Blotting

    Pages 33-45

    Casari, Erika (et al.)

  • Analysis of DNA Double-Strand Break End Resection and Single-Strand Annealing in S. pombe

    Pages 47-57

    Yan, Zhenxin (et al.)

  • Quantifying DNA End Resection in Human Cells

    Pages 59-69

    Zhou, Yi (et al.)

Buy this book

eBook $219.00
price for USA in USD
  • ISBN 978-1-0716-0644-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $279.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Homologous Recombination
Book Subtitle
Methods and Protocols
Editors
  • Andres Aguilera
  • Aura Carreira
Series Title
Methods in Molecular Biology
Series Volume
2153
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0644-5
DOI
10.1007/978-1-0716-0644-5
Hardcover ISBN
978-1-0716-0643-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 557
Number of Illustrations
35 b/w illustrations, 91 illustrations in colour
Topics

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