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The Plant Cell Wall

Methods and Protocols

  • Book
  • © 2020
  • Latest edition

Overview

Editors:
  1. Zoë A. Popper

    1. Botany and Plant Science School of Natural Sciences, Ryan Institute for Environmental Marine and Energy Research School of Natural Sciences, The National University of Ireland Galway, Galway, Ireland

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2149)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. High-Voltage Paper Electrophoresis (HVPE)

    • Stephen C. Fry
    Pages 1-31

  3. Carbohydrate Gel Electrophoresis

    • Florence Goubet, Paul Dupree, Katja Salomon Johansen
    Pages 33-44

  4. Capillary Electrophoresis with Detection by Laser-Induced Fluorescence

    • Andrew Mort, Xiangmei Wu
    Pages 45-56

  5. Theory and Practice in Measuring In-Vitro Extensibility of Growing Plant Cell Walls

    • Daniel J. Cosgrove
    Pages 57-72

  6. Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Komagataeibacter Fermentation

    • Deirdre Mikkelsen, Patricia Lopez-Sanchez, Dongjie Wang, Michael J. Gidley
    Pages 73-87

  7. Plant Tissue Cultures

    • Anna Kärkönen, Arja Santanen, Kuninori Iwamoto, Hiroo Fukuda
    Pages 89-109

  8. Protoplast Isolation and Manipulation in the Unicellular Model Plant Penium margaritaceum

    • David S. Domozych, Eleanore Ritter, Anna Lietz, Berke Tinaz, Sandra C. Raimundo
    Pages 111-124

  9. Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patens

    • Alison W. Roberts, Christos S. Dimos, Michael J. Budziszek Jr, Chessa A. Goss, Virginia Lai, Arielle M. Chaves
    Pages 125-144

  10. Expression of Cell Wall–Modifying Enzymes in Aspen for Improved Lignocellulose Processing

    • Marta Derba-Maceluch, Ewa J. Mellerowicz
    Pages 145-164

  11. Activity and Action of Cell-Wall Transglycanases

    • Lenka Franková, Stephen C. Fry
    Pages 165-192

  12. Screening for Cellulolytic Plant Enzymes Using Colorimetric and Fluorescence Methods

    • Kirsten Krause, Stian Olsen
    Pages 193-201

  13. Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls

    • Stefan J. Hill, Tracey J. Bell, Laurence D. Melton, Philip J. Harris
    Pages 203-223

  14. High-Resolution Imaging of Cellulose Organization in Cell Walls by Field Emission Scanning Electron Microscopy

    • Yunzhen Zheng, Gang Ning, Daniel J. Cosgrove
    Pages 225-237

  15. Analyzing Plant Cell Wall Ultrastructure by Scanning Near-Field Optical Microscopy (SNOM)

    • Tobias Keplinger, Ingo Burgert
    Pages 239-249

  16. Raman Imaging of Plant Cell Walls

    • Batirtze Prats Mateu, Peter Bock, Notburga Gierlinger
    Pages 251-295

  17. Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy

    • Ricardo M. F. da Costa, William Barrett, José Carli, Gordon G. Allison
    Pages 297-313

  18. Rapid Assessment of Cell Wall Polymer Distribution and Surface Topology of Arabidopsis Seedlings

    • Mary L. Tierney, Li Sun, David S. Domozych
    Pages 315-325

  19. Analysis of Plant Cell Walls Using High-Throughput Profiling Techniques with Multivariate Methods

    • John P. Moore, Yu Gao, Anscha J. J. Zietsman, Jonatan U. Fangel, Johan Trygg, William G. T. Willats et al.
    Pages 327-337

  20. Vibratome Sectioning of Plant Materials for (Immuno)cytochemical Staining

    • Olivier Leroux
    Pages 339-350
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Keywords

About this book

This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology. Chapters in this book also cover topics such as cell wall composition and structure, plant tissue culture protoplast isolation, genetic manipulation, investigation of enzyme activities, and in situ localization of wall components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and comprehensive, The Plant Cell Wall: Methods and Protocols, Second Edition is a valuable resource for both novice and expert scientists interested in learning more about this field. 

Editors and Affiliations

  • Botany and Plant Science School of Natural Sciences, Ryan Institute for Environmental Marine and Energy Research School of Natural Sciences, The National University of Ireland Galway, Galway, Ireland

    Zoë A. Popper

Bibliographic Information

  • Book Title: The Plant Cell Wall

  • Book Subtitle: Methods and Protocols

  • Editors: Zoë A. Popper

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0621-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0619-3Published: 03 July 2020

  • eBook ISBN: 978-1-0716-0621-6Published: 02 July 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVII, 544

  • Number of Illustrations: 39 b/w illustrations, 102 illustrations in colour

  • Topics: Plant Physiology, Plant Anatomy/Development, Plant Biochemistry, Plant Genetics and Genomics

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