Read While You Wait - Get immediate ebook access, if available*, when you order a print book

Springer Protocols Handbooks

CRISPR-Cas Methods

Editors: Islam, M. Tofazzal, Bhowmik, Pankaj, Molla, Kutubuddin A. (Eds.)

Free Preview
  • Includes cutting-edge methods and protocols
    Provides step-by-step detail essential for reproducible results
    Contains key notes and implementation advice from the experts

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-0716-0616-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-0716-0615-5
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
About this book

This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. 
Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.

About the authors

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Wide Horizons of CRISPR-Cas-Derived Technologies for Basic Biology, Agriculture, and Medicine

    Pages 1-23

    Molla, Kutubuddin A. (et al.)

  • Efficient Homologous Recombination-Mediated in Planta Gene Targeting by Egg-Cell-Specific Expression of Staphylococcus aureus Cas9 from Arabidopsis

    Pages 25-34

    Wolter, Felix (et al.)

  • Rice Gene Knockout or Downregulation through CRISPR-Cas9

    Pages 35-50

    Ludwig, Yvonne (et al.)

  • CRISPR-Cas-Mediated Single Base Editing at More than One Locus in Rice Genome

    Pages 51-62

    Molla, Kutubuddin A. (et al.)

  • CRISPR-Cas9-Mediated Genome Editing of the Model Grass Species Brachypodium distachyon

    Pages 63-86

    Petrik, Deborah

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-0716-0616-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-0716-0615-5
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
CRISPR-Cas Methods
Editors
  • M. Tofazzal Islam
  • Pankaj Bhowmik
  • Kutubuddin A. Molla
Series Title
Springer Protocols Handbooks
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0616-2
DOI
10.1007/978-1-0716-0616-2
Hardcover ISBN
978-1-0716-0615-5
Series ISSN
1949-2448
Edition Number
1
Number of Pages
XIV, 278
Number of Illustrations
9 b/w illustrations, 58 illustrations in colour
Topics

*immediately available upon purchase as print book shipments may be delayed due to the COVID-19 crisis. ebook access is temporary and does not include ownership of the ebook. Only valid for books with an ebook version. Springer Reference Works and instructor copies are not included.